Chitozen

Price range: $145.00 through $8,565.00

Chitozen in a chitosan-coated coverslip designed for immobilizing bacteria for microscopy without inducing bacteriostatic effects. Chitozen is for resesarch use only and not to be used diagnostic procedures.

SKU: TMI-CHI Category: Tags: ,

Chitozen

Kit Options

  • 5-coverslips alone*
    • 5x standard (25 x 75 mm) chitosan-coated coverslips
  • 5-coverslips with microfluidic channels (= 30 experiments)
    • 5x standard (25 x 75 mm) chitosan-coated coverslips
    • 5x bottomless 6-channel sticky slides
  • Centrifugation pack: the Chitozen coverslips can be centrifuged using a rack compatible with standard microscope slide (25 x 75 mm) dimensions. If needed, add our centrifugation pack to your Chitozen kit. It includes:
    • µ-Slide Microscopy Rack (ibidi)
    • Magnetic Lid for Microscopy Rack
    • Clamp & adapter to securely fasten sticky slides to Chitozen coverslips

*If using the Chitozen coverslips alone, separate wells can be created manually by using a sealing glue or Stencell silicon chambers. Contact us for more information.

Features:

  • Increased Stability: Maintain bacteria in a same focal plane for imaging while preserving their physiology
  • Capture the Moment: Fix your bacteria and label them in situ to look at specific protein expression patterns during dynamic processes
  • Renew Culture Medium and Change Growth Condition: Change experiment conditions and directly observe, in real-time, the bacteria new comportment under the microscope
  • Extensive Micro-Organism Compatibility
    • E.coli
    • Bacillus subtilis
    • Caulobacter crescentus
    • Corynebacterium glutamicum
    • Helicobacter pylori
    • Mycobacterium smegmatis
    • Myxococcus xanthus
    • Pseudomonas aeruginosa
    • Pseudomonas fluorescens
    • Salmonella
    • Staphylococcus aureus
    • Vibrio cholerae
    • Acanthamoeba castellanii

Applications:

  • Experimental Outputs
    • Behavioral changes: growth, elongation, cell division, fitness, colony/biofilm formation, predation, motility, etc.
    • Single molecule imaging
  • Assay Compatiblity
    • Live imaging
    • Co-cultures (bacterial predators, immune cells)
    • Additon of external factors (e.g. antibiotics, chemicals, inhibitors)
    • Static or dynamic conditions
    • Immunostaining procedures
  • Imaging Modes
    • Phase-contrast
    • Epifluorescence
    • Confocal
    • Super-resolution microscopy
    • Atomic force microscopy – documentation & example pictures available on request

This Chitozen is manufactured in France by Idylle SAS.

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