The ExactiFi Hot-Start DNA Polymerase (1 U/µl) is a highly purified thermostable recombinant proofreading DNA polymerase. The ExactiFi Hot-Start DNA Polymerase (1 U/µl) is for research use only and not to be used for diagnostic procedures.
The enzyme catalyzes template-dependent nucleotide polymerization in the 5’→3′ direction. Additionally, the 3’→5′ exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5’→3′ exonuclease activity and no detectable reverse transcriptase activity, producing blunt-end PCR products.
This ExactiFi Hot-Start DNA Polymerase is manufactured in Germany by biotechrabbit.
Size
100 units
Component
HiFi Hot-Start DNA Polymerase – HiFi Hot-Start DNA Polymerase, 1 U/µl, in storage buffer containing 50 % (v/v) glycerol
HiFi Reaction Buffer – Optimized reaction buffer without magnesium ions
dNTP Mix (10 mM each) – Aqueous solution (pH 7.0) containing 10 mm each: dATP, dCTP, dGTP, dTTP sodium salts
MgCl2 – 50 mM MgCl2 in water
Application
High-fidelity PCR, Generation of PCR products for blunt cloning
