Cetuximab (ERBITUX) ELISA Assay Kit
The Cetuximab (ERBITUX) ELISA Assay Kit is For Research Use Only
Size: 12 x 8 wells
Sensitivity: 0.03 ug/mL
Standard Range: 0 – 300 ug/mL
Incubation Time: 1 hour 10 minutes
Sample Type: Serum, Plasma
Sample Size: 10 µL
Alternative Name: Erbitux
Assay Background
Cetuximab is an epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions. Cetuximab binds to the epidermal growth factor receptor (EGFr) on both normal and tumor cells. EGFr is over-expressed in many colorectal cancers. Cetuximab competitively inhibits the binding of epidermal growth factor (EGF) and TGF alpha, thereby reducing their effects on cell growth and metastatic spread.
Assay Procedure
Solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. Standards and samples (serum or plasma) are incubated in the microtiter plate coated with the reactant for cetuximab. After incubation, the wells are washed. Then, horse radish peroxidase (HRP) conjugated probe is added and binds to cetuximab captured by the reactant on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of tetramethylbenzidine (TMB) chromogen substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of cetuximab in the sample or standard. Results of samples can be determined directly using the standard curve.
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