Salmon/Trout IGF-II Polyclonal Antibody (Rabbit)
The Eagle Bioscience’s Salmon/Trout IGF-II Polyclonal Antibody (Rabbit) is for research use only and not to be used for diagnostic procedures.
Product Specifications
Source:
HCIC purified IgG from rabbit antiserum (100 μl). Each vial contains approximately 520 μg immunoglobulin.
Description:
Salmon/Trout IGF-II polyclonal antibody was prepared by immunising rabbits with a synthetic peptide epitope corresponding to amino acid residues 1-9 of the N-terminus of mature Atlantic salmon (Salmo salar) IGF-II. The antiserum was harvested and the IgG fraction partially purified by hydrophobic charge induction chromatography. The antibody is highly specific for IGF-II from Atlantic salmon and Rainbow trout (Oncorhynchus mykiss) in an enzyme-linked immunoassay. It has a cross-reactivity of ~ 5% to salmon insulin and < 0.01% to salmon IGF-I and salmon GH.
- Quantity: 100 µl
- Immunogen: Derived from UniProtKB/Swiss-Prot sequence ID A7LBA7 Atlantic salmon IGF-II. The peptide sequence is amino-acids 11-19 (IGF-II1-9) conjugated to diphtheria toxoid.
- Source: HCIC purified IgG from rabbit antiserum (100 μl). Each vial contains approximately 520 μg immunoglobulin.
- Specificity: .6 % cross-reactivity with salmon insulin < 0.1 % cross-reactivity with salmon/trout IGF-I (Oncorhynchus kisutch & Oncorhynchus mykiss), salmon/trout growth hormone, tuna IGF-I (Thunnus maccoyii), tuna IGF-II, bream IGF-I (Pagrus auratus), bream growth hormone (Acanthopagrus butcheri), barramundi IGF-I (Lates calcarifer), human IGF-I, human IGF-II, rat IGF-I, rat IGF-II, rat growth hormone.
- Appearance: Lyophilized powder
- Storage/Stability: At least 2 years at 2 – 4°C (lyophilized). After reconstitution store at -20°C or -80°C. Avoid freeze-thaw cycles
- Reconstitution: Dissolve the vial contents in 500μl buffer suitable for enzyme-linked immunoassay applications.
- Application & Titre: Each application and titre should be determined in house but as a guideline: Enzyme immunoassay 1:1,000 Immunoblotting 1:1,000
Related Products
Human IGF-II (Receptor Grade)
Mono-biotinyl Human IGF-II
Human IGF-I Monoclonal Antibody
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