EasYeast

EasYeast

Price range: $530.00 through $1,470.00

EasYeast is designed for yeast DNA extraction. It is based on an innovative lysis buffer optimized to efficiently break up yeast cell wall at moderate temperatures without the need for harmful organic solvents, expensive enzymes or mechanical rupture. EasYeast is for research use only and not to be used for diagnostic procedures.

SKU: IDY-EY Category: Tags: ,

EasYeast

Kit Contents

  • Small Kits: 300 extractions
    • 1.2 mL Lysis buffer 10X
    • 1.2 mL Stop buffer 10X
  • Large Kits: 1,000 extractions
    • 4 mL Lysis buffer 10X
    • 4 mL Stop buffer 10X

Features:

  • Go faster: Only 2 ready-to-use solutions, 3 simple steps, and a total time under 1 hour. The most straight-forward method for yeast DNA extraction
  • Get high-quality DNA: With no SDS, phenol/chloroform or boiling steps, the EasYeast method best preserves the extracted DNA integrity and minimizes the risk of recovering contaminants that may interfere with downstream applications. No need for purification columns, use it straight away for PCR or bacterial amplification
  • Scale-up your DNA extraction process: Based solely on non-toxic aqueous solutions, EasYeast does not require the use of bead beating, columns or sophisticated equipments. The extraction protocol can be easily implemented in 96-well plate formats or automatized and is your solution of choice where large numbers of samples need to be extracted.

Applications:

EasYeast is polyvalent: only 1 kit covering all your yeast experiments

  • Quickly extract extra-chromosomic DNA (i.e. plasmid, YAC) directly usable for PCR amplification and bacterial transformation (cloning/subcloning, recombinant protein expression, library screening, double-hybrid assays, etc).
  • A method of choice for a cost-effective extraction of genomic DNA suitable for PCR amplification. Compatible with affinity-based purification methods, EasYeast can also be easily integrated within existing experimental workflows when ultra-pure DNA is needed.

Storage

The Lysis buffers, as they are or diluted to 1X, should be stored at -20°C if not used immediately.
The 10X Stop buffer can be stored at room temperature for up to 2 months. For long-term storage, store at -20°C.
Both buffers can be stored at -20°C for a year.

This EasYeast is manufactured in France by Idylle SAS.

Size
Sensitivity
Dynamic Range
Incubation Time
Sample Type
Storage -20°C
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