Androstenedione is a steroid hormone produced primarily by the adrenal glands and gonads and serves as a direct precursor to both testosterone and estrone. In females, the ovaries contribute significantly to circulating androstenedione levels, which can fluctuate throughout the ovulatory cycle. Because of its central role in the steroidogenesis pathway, measuring serum androstenedione is crucial for evaluating adrenal and ovarian function, especially in cases of suspected hormonal imbalance or androgen excess.
Clinically, androstenedione is an important biomarker for diagnosing and monitoring disorders related to androgen production. In women, abnormal testosterone levels—such as those seen in conditions like polycystic ovary syndrome (PCOS), adrenal hyperplasia, or androgen-secreting tumors—should be evaluated alongside serum androstenedione to help identify whether the source of excess androgen is adrenal or ovarian in origin. This dual measurement approach enhances diagnostic accuracy and aids in tailoring appropriate treatment strategies.
The Androstenedione ELISA Assay Kit offers a competitive immunoassay format for the sensitive quantification of androstenedione in serum samples. During the assay, sample-derived androstenedione competes with an HRP-labeled conjugate for binding to specific antibodies coated on microplate wells. After unbound materials are washed away, a TMB substrate is added, generating a color signal inversely proportional to the amount of androstenedione in the sample. Absorbance is measured at 450 nm, and the hormone concentration is determined using a standard curve. This assay supports both clinical diagnostics and research into reproductive endocrinology and androgen-related conditions.
This product is manufactured in USA by Eagle Biosciences.