Adrenocorticotropic Hormone (ACTH) ELISA Kit

$500.00

The Eagle Biosciences Human Adrenocorticotrophic Hormone (ACTH) ELISA Kit is intended for the quantitative determination of human Adrenocorticotrophic Hormone (ACTH) levels in plasma. The Eagle Biosciences Human Adrenocorticotrophic Hormone (ACTH) ELISA Kit is for research use only and not to be used in diagnostic procedures.

SKU: ACT31-K01 Category: Tags: , , ,

Adrenocorticotropic Hormone (ACTH) is a 39-amino acid polypeptide hormone with a molecular weight of approximately 4540 Daltons. It is synthesized by corticotrope cells in the anterior pituitary gland in response to corticotropin-releasing hormone (CRH) from the hypothalamus. ACTH originates from the precursor molecule pre-pro-opiomelanocortin (pre-POMC), which is first translated into pro-opiomelanocortin (POMC), a 241-amino acid polypeptide. POMC is then processed through phosphorylation, glycosylation, and proteolytic cleavage into biologically active peptides, including ACTH.

ACTH plays a central role in the hypothalamic-pituitary-adrenal (HPA) axis, particularly in stress response. It stimulates the adrenal cortex—specifically the zona fasciculata—to secrete glucocorticoid hormones such as cortisol. These hormones are essential for regulating metabolism, immune response, and maintaining homeostasis during physiological stress. ACTH achieves this by binding to specific receptors (MC2R) on the surface of adrenocortical cells.

In clinical and research settings, measurement of ACTH levels is used to evaluate disorders of adrenal function, including Cushing’s syndrome, Addison’s disease, and secondary adrenal insufficiency. ACTH testing is also valuable in differentiating between adrenal and pituitary causes of cortisol imbalance and in assessing the integrity of the HPA axis during dynamic stimulation tests.

This product is manufactured in USA by Eagle Biosciences.

Size 1 x 96 Well
Sensitivity 1 pg/mL
Dynamic Range 11 – 416 pg/mL
Incubation Time 2 hours 20 minutes
Sample Type EDTA-Plasma
Immunogen
Source Species
Specificity
Expression System
Clone
Group
Alpha Chain
Beta Chain
Peptide
Peptide Source
Format
Buffer
Concentration
Storage 2-8°C
Alternative Names Adrenocorticotropic hormone, ACTH, Corticotropin, Adrenocorticotrophin, Adrenocorticotrophic hormone, POMC-derived peptide, Pituitary adrenotropic hormone, Hypophyseal corticotropic hormone, Corticotropic hormone, 39-amino acid ACTH.
Instructions For Use https://eaglebio.com/wp-content/uploads/2025/06/ACT31-K01_ACTH_ELISA_Kit_IFU.pdf
MSDS https://eaglebio.com/wp-content/uploads/2014/06/ACT31-K01-ACTH-ELISA-Assay-Kit-MSDS.pdf

Product Citations