Eagle Biosciences Welcomes Another Product to the Endocrinology ELISA Product Line!
A Little Bit About Pancreatic Elastase…
Pancreatic elastase is an anionic endoprotease of the serine protease family with a molecular weight of 26 kDa. Together with other digestive enzymes it is synthesized as an inactive pro-enzyme in the acinar cells of the pancreas and is secreted into the duodenum. After its activation, pancreas elastase cleaves peptides after neutral amino acids. Pancreas elastase is mainly bound to bile salts during intestinal passage and is not degraded. In human feces it is 5–6 fold more concentrated than in pancreatic juice. The stool concentration reflects the secretory capacity of the pancreas.
What You Can Expect…
The Eagle Biosciences Pancreatic Elastase ELISA Assay Kit is intended for the quantitative determination of human pancreatic elastase in stool. This kit is for research use only and should not be used in therapeutic procedures. The Eagle Biosciences Pancreatic Elastase ELISA Assay Kit is intended for the quantitative determination of pancreatic elastase in stool. In a first incubation step, the pancreatic elastase in the samples is bound to monoclonal antibodies, immobilized to the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a peroxidase-labeled conjugate (mouse anti pancreatic elastase) is added which recognizes specifically the bound pancreatic elastase. After another washing step to remove all unbound substances, the solid phase is incubated with the substrate, tetramethyl-benzidine (TMB), which reacts with the peroxidase. An acidic stop solution is added to stop the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of pancreatic elastase. A dose response curve of absorbance unit (optical density, OD at 450 nm) vs. concentration is generated using the values obtained from the standards. Pancreatic elastase present in the patient samples is determined directly from this curve.
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