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Eagle Biosciences’ Steroid Assay Kit line offers an array of interesting and sensitive assays for different hormone analytes and research needs. OurFree Testosterone ELISA is a top seller at Eagle Biosciences and continues to be a valuable tool for researchers in various fields of study. It offers a variety advantages over other kits in the market and it is recognized for its ability to measure the free or unbound fraction of serum testosterone with unsurpassed sensitivity and accuracy.

Why Measure Free Testosterone with EagleBio’s Kit?

1. Excellent Specificity: 100% specificity to free testosterone

2. Unsurpassed Sensitivity: lowest detectable concentration is 0.04 pg/ml

3. Quick and Simple Test Results: < 2hours

4. Easy to Automate

Our Free Testosterone ELISA Kit is a 2nd generation assay and is more sensitive, accurate, and precise than the previous versions with less interference of matrix effects in samples. It provides an excellent correlation to free androgen index (total Testosterone/SHBG) and is an effective means to truly capture the unbound or free testosterone in each sample thereby estimating the physiologically bioactive hormone.

Related Kits:

Steroid Assay Kits

Testosterone ELISA Assay Kit

Testosterone Saliva ELISA Assay Kit

Related News:

Eagle Biosciences Shares New Literature Utilizing Select Hormone Assays


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A recent study by Chinese scientists involving the attempt to modify the genes of human embryos has sparked some controversy and some concern by other scientists in the field.  The process they used was a genetic engineering technique called CRISPR (clustered regularly interspaced short palindromic repeat) to remove a faulty gene and replace it with a healthy one in human embryos.  The original plan and hope for such a process may hold extraordinary promise for both basic research and clinical applications. However, DNA repair mechanisms have not been studied enough and are not fully understood, thus it is difficult for researchers to predict how the genome will be affected and what mutations could occur.  Looking at the bigger picture beyond the potential power of treating diseases, the unpredictable effects on future generations places this process in jeopardy of being defined as unethical and dangerous. Read More


What do you think?

image above from www.livescience.com

EagleBio Spotlight: Free Testosterone



Testosterone
is a steroid hormone from the
androgen group and is predominantly secreted in the testes of males and the
ovaries of females however, there is a small amount of this hormone that is secreted
by the adrenal glands. Testosterone is
the primary male sex hormone as well as an anabolic steroid and it plays key
roles for the health and well-being of both males and females.

Only a small percentage of circulating testosterone
exists as unbound or free testosterone. In fact, testosterone circulates 98%
protein-bound (54% with low affinity to albumin and other proteins, and 44%
with high affinity to SHBG) with only 2% circulating as free testosterone. The
portion which is unbound, referred to as free testosterone, is considered the
component that has the ability to enter cells and in turn, has androgenic
effects. Thus, for these reasons it is proposed that free testosterone will
relate to the biological effect on tissue more than the total testosterone.


Reasons to
Measure Free Testosterone

It is important to measure Free Testosterone for detecting
and monitoring various conditions and diseases that affect both men and women.
Free testosterone is responsible for sexual traits and health problems can
result from elevated or decreased levels of this hormone. As mentioned above, the measurement of the
free or unbound fraction of serum testosterone provides a means of estimating
the physiologically bioactive hormone.


Free Testosterone has been associated
with many diseases and disorders/conditions

Males:

Primary and secondary hypogonadism- A failure of the gonads, testes in men and ovaries in
women, to function properly (i.e. primary– direct failure of gonad and secondary
indirect failure of gonad due to problem in the hypothalamus or the pituitary
gland)

Delayed or advanced puberty

Impotence

Loss of sex drive

Bone loss

Infertility

Cushing Syndrome


Females:

Hirsutism– excessive
hair

Virilization-
biological development of sex differences- changes that make the female body
different from a male body- masculization occurs) due to tumors

Polycystic ovaries –Polycystic ovary syndrome (PCOS)
a condition discerned by infertility, lack of menstruation, acne, and excessive
hair growth, particularly on the face

Androgenital syndromes

Cushing Syndrome


References:

1. Alan, Carolyn et. al. “Age-related changes in testosterone and the role of replacement therapy in older men.” Clinical Endocrinology. 2004; 60:6:653–670. 

2.Rosner W, Auchus RJ, Azziz R, et al. “Position statement: Utility, limitations, and pitfalls in measuring testosterone: an Endocrine Society Position Statement”. J Clin Endocrinol Metab. 2007;92:405-413. 

3. Sodergard, R et al. “Calculation of free and bound fractions of testosterone and estradiol-17 beta to human plasma proteins at body temperature”. Journal of Steroid Biochemistry. 1982; 16, 801–810. 


Related Kits:

Steroid Assay Kits

Free Testosterone ELISA Assay Kit

Testosterone ELISA Assay Kit

Testosterone Saliva ELISA Assay Kit

Related News:

Eagle Biosciences Shares New Literature Utilizing
Select Hormone Assays

Eagle Sciences offers a wide range of hormone assays that are the best kits available worldwide. Listed below are some new publications from scientists who have successfully completed their studies utilizing our: Estradiol ELISA, Testosterone ELISAProgesterone ELISA, and Cortiol Saliva ELISA kits from our Steroid Assay Kit Line.



Estradiol ELISA Assay Kit,Testosterone ELISA Assay KitProgesterone ELISA Assay Kit:

New Publication:

Investigation of sex reversal in layer chickens in Bangladesh


Cortisol Saliva ELISA Assay Kit:

New Publication:

Circadian rhythms of ewes suckling singletons versus twins during the second week of lactation

Related Kits:

Steroid Assay Kits


Related News:

April 1, 2011: Eagle Biosciences Introduces High Sensitive Steroid Saliva ELISA Kits



Sharing a makeup brush with your best friend seems harmless right? Well under ordinary circumstances the answer is yes but for Jo Gilchrist it caused an absolutely devasting outcome for her. She contracted a serious MRSA infection that attacked her spine that ultimately paralyzed her.

Most people do have staphylococcus bacterial species as part of their normal skin flora but there is a small percentage of people that carry a very antibiotic-resistant strain called MRSA (a.k.a. Methicillin-resistant Staphlococcus aureus). Jo’s friend had previously had a staph infection on her face and doctors believe that Jo was infected with MRSA through a pimple on her face.after using the borrowed makeup brush.

Learn More


makeup image above from www.abc22now.com

Related News

Magic, Ancient Concoction Kills Today’s Superbugs

Eagle Biosciences Adds New Assays for CD Antigens, Soluble Antigens, Cytokine Receptors, and Apoptosis Markers


EagleBio is excited to share a list of numerous, new assays for measuring cytokines,CD antigens, and apoptosis markers. They assays have been added to our immunology assay kit line and they offer broad antibody range across multiple species with high specificity and affinity for a number of different cytokines and soluble molecules.  They recognize both natural and recombinant antigen specificity with no cross reactivity with other human cytokines.


Please see these new Eagle Biosciences Assays below:


Human CD14 ELISA Assay Kit

Human sCD141 ELISA Assay Kit

Human CD21 ELISA Assay Kit

Human CD86 ELISA Assay Kit

Human CD116 ELISA Assay Kit

Human CD117 ELISA Assay Kit

Human CD124 / IL4-R ELISA Assay Kit

Human CD126 / IL-6R ELISA Assay Kit

Human CD130 / gp130 ELISA Assay Kit

Human CD138 /SYNDECAN-1 ELISA Assay Kit

Human CD213α2 / IL-13Rα2 ELISA Assay Kit

Human sCD25 / IL-2R ELISA Assay Kit

Human CD102 / sICAM-2 ELISA Assay Kit

Human CD106 / sVCAM-1 ELISA Assay Kit

Human CD31 ELISA Assay Kit

Human CD44 ELISA Assay Kit

Human CD50 / sICAM-3 ELISA Assay Kit

Human CD54 ELISA Assay Kit

Human CD62E ELISA Assay Kit

Human CD62L ELISA Assay Kit

Human CD62P ELISA Assay Kit


Related Products:

Cytokine ELISA Assay Kits

Endocrinology Assay Kits

New Products


Related News:

Eagle Biosciences Launches New High Sensitivity Cytokine Assays 

Eagle Biosciences Announces the Launch of Multispecies 25-OH Vitamin D ELISA Assay Kit



Eagle Biosciences, Inc. is happy to announce the product launch of a new Multispecies 25-OH Vitamin DELISA kit. This will be one of the first commercially available ELISA kits to measure both 25-OH Vitamin D2 and D3 equally, at one hundred percent in multiple species such as bovine, goat, horse, chicken, mouse, rat, rabbit and equine. Millions of tests are performed annually for vitamin D and recent research has proven that the only way to accurately assess vitamin D status is to measure both circulating forms.

Dan Keefe, President of Eagle Biosciences, is very pleased with this unique Multispecies 25-OH Vitamin D ELISA. “We are thrilled for the opportunity to offer this expedient and extremely versatile kit for vitamin D determination.” Keefe continued, “This assay is an amazing addition to our ever growing vitamin assay kit product line and is a perfect complement to the rock solid, gold standard assay we offer, 25-OH Vitamin D ELISA kit (for Humans).”

Keefe concluded, “This assay was carefully designed with extensive optimization and numerous validation studies. In fact, one of the key steps in the development involved utilizing our own assay buffer that releases Vitamin D from Vitamin D binding protein (DBP). This buffer’s unique properties offer researchers the ability to run samples without sample extraction. Therefore the lack of extraction coupled with the short two hour incubation time makes this ELISA a very robust and easy assay to run.”

This product is currently for research use only. Eagle Biosciences will be offering promotional pricing through the end of May for anyone who purchases the Multispecies 25-OH Vitamin D ELISA. Visit the website at www.EagleBio.com or call 866-411-8023 for full details.


Related Products:

Vitamin Assay Kits

Related News:

New Publications for EagleBio’s Vitamin Assay Kit Product Line

EagleBio Spotlight: Vitamin D

Interesting Study Reveals Possible Link Between Vitamin D and Autism

New Publications for Select Vitamin Assays Kits

EagleBio’s 25-OH Vitamin D ELISA: Product Highlights

10 Reasons to Keep Up with Your Vitamin D Levels


Monthly Promotions- Multispecies 25-OH Vitamin D ELISA



Check Out our Monthly Promotions!! 

40% Discount on our New Multispecies 25-OH Vitamin D ELISA Kit

This promotion is good through the end of May just use the code below when ordering.

PROMOTIONAL CODE: VDM0540




Related Products:

Vitamin Assay Kits

Related News:

Eagle Biosciences Announces the Launch of Multispecies 25-OH Vitamin D ELISA Assay Kit

New Publications for EagleBio’s Vitamin Assay Kit Product Line

EagleBio Spotlight: Vitamin D

Interesting Study Reveals Possible Link Between Vitamin D and Autism

New Publications for Select Vitamin Assays Kits

EagleBio’s 25-OH Vitamin D ELISA: Product Highlights

10 Reasons to Keep Up with Your Vitamin D Levels

New Publications for EagleBio’s Vitamin Assay Kit Product Line



EagleBio offers numerous unique and robust ELISAs, HPLCs, antigens, antibodies, proteins, and more in a variety of emerging areas of research and disciplines. The Vitamin Assay Kit line offered Eagle Biosciences provides unique and sensitive assays for measuring a variety of vitamin analytes.  Researchers in the field are continually accomplishing fascinating studies and using our kits to do so!  Below you will find two new publications referencing 25-OH Vitamin D ELISA Assay Kit and Vitamin A / Vitamin E HPLC Assay Kit respectively.


25-OH Vitamin D ELISA Assay Kit

Vitamin A / Vitamin E HPLC Assay Kit

New Publication:

Determination of Vitamins A, C and D Status Using Serum Markers and a 24-Hour Dietary Recall among Maasai Women of Reproductive Age


Related Products:

Vitamin Assay Kits

Related News:

EagleBio Spotlight: Vitamin D

Interesting Study Reveals Possible Link Between Vitamin D and Autism

New Publications for Select Vitamin Assays Kits

10 Reasons to Keep Up with Your Vitamin D Levels

Eagle Biosciences Launches New High Sensitivity Cytokine Assays


EagleBio is proud to announce the new launch of three new high sensitivity cytokine assays.  These kits offer ultra-sensitivity, excellent specificity with no cross reaction to other cytokines, and the ability to test multiple sample types. Please see these new Eagle Biosciences Assays below:


Human High Sensitive IFNγ ELISA Assay Kit

Interferon-gamma (also known as: IFN-gamma and Type II interferon or immune interferon) is a cytokine that plays physiologically important roles in promoting innate and adaptive immune responses. 


Sample types: Human Sera, Plasma, Buffered Solutions or Cell Culture Media

Detects: Both Natural and Recombinant Human IFNγ


Human IL-2 High Sensitivity ELISA Assay Kit

Interleukin 2 (IL-2is a cytokine cell signalling molecule of the immune system which is considered a key growth and death factor for antigen-activated T lymphocytes. IL-2 plays a vital role in lymphocyte differentiation, immune responses, and homeostatis.


Sample Types: Human Sera, Plasmas, Buffered Solutions or Cell Culture Media. 

Detects: Both Natural and Recombinant Human IL-2


Human IL-6 High Sensitivity ELISA Assay Kit

Interleukin 6 (IL-6) is a pro-inflammatory cytokine and an anti-inflammatory myokine that is not involved in inflammation and infection responses as well as regulation of metabolic, regenerative, and neural processes. IL-6 is released by T cells and macrophages and is a vital component in the process of stimulating an immune response.


Sample Types: Human Sera, Plasmas, Buffered Solutions, Supernatants, and other Body Fluids. 

Detects: Both Natural and Recombinant Human IL-6



Related Products:

Cytokine ELISA Assay Kits

Endocrinology Assay Kits

New Products