Serotonin (5-hydroxytryptamine) is a biogenic amine derived from tryptophan metabolism and functions as a key neurotransmitter in the central nervous system. It is also found in high concentrations in the intestinal mucosa’s chromaffin cells, blood platelets, and serotonergic neurons in the brain. Serotonin plays an essential role in regulating sleep, mood, cardiovascular activity, and hormonal balance. Clinically, abnormal serotonin levels are associated with a range of disorders, including depression, schizophrenia, and particularly malignant carcinoid tumors, for which it serves as a specific tumor marker.
In research and clinical settings, serotonin is measured to evaluate neurochemical balance, diagnose neuroendocrine tumors, and monitor psychiatric or metabolic disorders. Increased serum serotonin levels can be indicative of carcinoid syndrome, while altered levels in urine and serum have been observed in mood disorders and other neurological conditions. These applications make serotonin a critical biomarker for both diagnostic and monitoring purposes across a wide range of disciplines.
The Serotonin ELISA kit offers a reliable method for quantifying derivatized serotonin in human serum and urine. During sample preparation, serotonin is converted into N-acylserotonin using an acylating reagent. The competitive immunoassay format allows for sensitive detection by measuring the inverse relationship between serotonin concentration and signal intensity through a peroxidase-mediated colorimetric reaction using TMB. This assay supports both clinical diagnostics and biomedical research by providing accurate measurement of serotonin activity in physiological and pathological states.
This product is manufactured in Germany by DLD Diagnostika.