RTS Linear Template Kit Plus
The kit contains primers providing regulatory elements required for optimal transcription and translation in cell-free expression systems. Specially designed 5′-untranslated regions reduce the formation of secondary structure in the translation initiation region, one of the most common causes of low expression rates. A His-tag or Strep-tag II can be added to either terminus, greatly simplifying protein purification and detection. A signal sequence enables expression of functional proteins containing disulfide-bonds or membrane proteins in RTS 100 Insect Membrane or Disulfide kits. A signal peptide can be introduced using the N-terminal primer, which contains a melittin sequence. This enables protein translocation into microsomal vesicles derived from insect cell endoplasmic reticulum.
This RTS Linear Template Kit Plus is manufactured in Germany by biotechrabbit.
| Size | 20 PCRs of 25 µL |
| Application | Fast generation of multiple template constructs, Accelerated screening of protein expression templates and conditions |
| Hot Start | No |
| Storage | -20°C |
