RTS Linear Template Kit Plus

$520.00

The RTS Linear Template Kit Plus accelerates the generation of multiple templates for parallel protein expression. Constructs are suitable for expression in E. coli and insect cell-free lysates. Due to compatible restriction sites in the adapter primers, products can be easily cloned into pIX3.0 vectors for large-scale in vitro expression. The RTS Linear Template Kit Plus is for research use only and not to be used for diagnostic procedures.

SKU: BR1402401 Category: Tags: ,

RTS Linear Template Kit Plus

The kit contains primers providing regulatory elements required for optimal transcription and translation in cell-free expression systems. Specially designed 5′-untranslated regions reduce the formation of secondary structure in the translation initiation region, one of the most common causes of low expression rates. A His-tag or Strep-tag II can be added to either terminus, greatly simplifying protein purification and detection. A signal sequence enables expression of functional proteins containing disulfide-bonds or membrane proteins in RTS 100 Insect Membrane or Disulfide kits. A signal peptide can be introduced using the N-terminal primer, which contains a melittin sequence. This enables protein translocation into microsomal vesicles derived from insect cell endoplasmic reticulum.

This RTS Linear Template Kit Plus is manufactured in Germany by biotechrabbit.

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Storage -20°C
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