Pfu DNA Polymerase

Price range: $120.00 through $425.00

The Pfu DNA Polymerase is a highly purified thermostable recombinant proofreading DNA polymerase. The Pfu DNA Polymerase is for research use only and not to be used for diagnostic procedures.

SKU: BR03001 Category: Tags: ,

Pfu DNA Polymerase

The Pfu DNA Polymerase catalyzes template-dependent nucleotide polymerization in the 5’→3′ direction. Additionally the 3’→5′ exonuclease (proofreading) activity corrects nucleotide incorporation errors, thereby increasing fidelity and accuracy of DNA polymerization. The enzyme has no 5’→3′ exonuclease activity and no detectable reverse transcriptase activity and produces blunt-end PCR products.

Unit Definition: One unit catalyzes the incorporation of 10 nmol of deoxyribonucleotides into a polynucleotide fraction in 30 minutes at 72°C.

This Pfu DNA Polymerase is manufactured in Germany by biotechrabbit.

Size 100 Units|500 Units
Component
  • Pfu DNA Polymerase – Pfu DNA Polymerase, 2.5 U/µl, in storage buffer containing 50% (v/v) glycerol
  • 10× Pfu Reaction Buffer – Optimized PCR reaction buffer including magnesium ions
  • 5× PCR Enhancer – Proprietary PCR enhancer mix
Application High-fidelity PCR, Generation of PCR products for blunt cloning, Site directed mutagenesis

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