Histamine is a biologically active amine formed by the decarboxylation of the amino acid histidine. It plays a central role in both normal physiological processes and pathological responses. As part of the immune system, histamine contributes to the body’s defense against perceived foreign substances and is a key mediator in allergic reactions, asthma, and other inflammatory responses. Upon activation, mast cells and basophils release histamine, triggering symptoms such as vasodilation, increased vascular permeability, and smooth muscle contraction.
In research and clinical settings, histamine is widely studied as a biomarker for allergic and inflammatory conditions. Its levels can be measured in various biological matrices to assess histamine-mediated activity and disease severity. The Histamine ELISA Kit enables the quantitative determination of derivatized histamine in human urine, EDTA and heparin plasma, whole blood, and stool samples. During sample preparation, histamine is converted into N-acylhistamine through derivatization, allowing for accurate detection in complex biological fluids.
This competitive enzyme immunoassay measures histamine levels based on the inverse relationship between antigen concentration and signal intensity, using a peroxidase-TMB colorimetric detection system. The assay’s broad sample compatibility and high sensitivity make it a valuable tool for diagnosing allergic conditions, monitoring treatment efficacy, and conducting immunological or pharmacological research related to histamine release and metabolism.
This product is manufactured in Germany by DLD Diagnostika.