Human growth hormone (hGH) is a 191-amino-acid polypeptide secreted by the somatotroph cells of the anterior pituitary gland. It plays a crucial role in regulating growth and metabolism, with primarily anabolic effects. hGH promotes protein synthesis, enhances glucose transport, facilitates glycogen storage, and stimulates the release of somatomedins (insulin-like growth factors), which further mediate tissue growth and development. Due to its central role in growth regulation, hGH is a key biomarker in evaluating developmental disorders and endocrine function.
Clinically, the measurement of hGH is most often used to diagnose and monitor disorders related to growth hormone secretion. In children, GH deficiency can result in growth retardation, while excess secretion causes gigantism. In adults, overproduction of GH leads to acromegaly, characterized by abnormal bone and tissue growth. Because GH is secreted in a pulsatile manner and fluctuates throughout the day, dynamic testing protocols—such as stimulation or suppression tests—are often required to accurately assess GH status rather than relying on a single measurement.
The Growth Hormone (GH) ELISA Assay Kit is a highly specific, one-step sandwich immunoassay designed to quantify GH in serum samples. It uses two monoclonal antibodies: one immobilized on the microplate to capture GH and another conjugated to HRP for detection. When both antibodies bind to GH, a sandwich complex is formed. After washing away unbound materials, a TMB substrate is added and the resulting color change is measured at 450 nm. The intensity of the signal is directly proportional to the amount of GH in the sample, enabling precise and reliable quantification for both clinical diagnostics and endocrine research.
This product is manufactured in USA by Eagle Biosciences.
Size | 1 x 96 Well |
Sensitivity | 0.2 ng/mL |
Dynamic Range | 1–50 ng/mL |
Incubation Time | 1.25 hours |
Sample Type | Serum |
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Storage | 2-8°C |
Alternative Names | Growth Hormone, GH, human growth hormone, hGH, somatotropin, somatotropic hormone, anterior pituitary hormone, anabolic growth factor, pituitary growth factor, GH 1 (gene product). |
Instructions For Use | https://eaglebio.com/wp-content/uploads/2025/05/RUO-HGH31-K01-Growth-Hormone-hGH-ELISA-Kit-IFU.pdf |
MSDS | https://eaglebio.com/wp-content/uploads/2014/06/EagleBio-SDS-ELISA-v6.0-718.pdf |