Dehydroepiandrosterone sulfate (DHEAS) is a sulfated steroid hormone primarily produced by the adrenal glands and, to a lesser extent, the gonads. It functions as a precursor to androgens such as testosterone and estrogens like estrone, playing a central role in steroid hormone biosynthesis. In women, both the adrenal glands and ovaries contribute to circulating DHEAS levels, which may fluctuate throughout the ovulatory cycle. Because of its origin and function, serum DHEAS is a valuable biomarker for evaluating adrenal and gonadal function, especially in the context of hormonal imbalances.
Clinically, DHEAS measurement is particularly useful in the assessment of hyperandrogenism in females. When elevated testosterone levels are detected, concurrent measurement of DHEAS helps differentiate whether the excess androgen originates from the adrenal glands or the ovaries. This distinction is crucial in diagnosing conditions such as adrenal tumors, polycystic ovary syndrome (PCOS), and congenital adrenal hyperplasia. By evaluating both testosterone and DHEAS levels, clinicians can more accurately pinpoint the source of endocrine dysfunction.
The DHEAS ELISA Assay Kit provides a sensitive and specific method for measuring DHEAS concentrations in serum using a competitive immunoassay format. In this assay, endogenous DHEAS competes with an HRP-labeled conjugate for binding to anti-DHEAS antibodies coated on microplate wells. After washing to remove unbound material, a TMB substrate is added, producing a color change inversely proportional to DHEAS concentration. Absorbance is measured at 450 nm, and concentrations are calculated via a standard curve. This method supports both clinical diagnostics and research applications related to endocrine and reproductive health.
This product is manufactured in USA by Eagle Biosciences.