Androstenedione Saliva ELISA Kit

Androstenedione is a steroid hormone produced primarily in the adrenal glands, ovaries, and testes, and it serves as an essential precursor in the biosynthesis of both testosterone and estrone. As an intermediate in the steroidogenic pathway, its circulating levels reflect upstream adrenal activity as well as downstream androgen and estrogen balance. Because androstenedione production is influenced by ACTH stimulation, ovarian theca cell function, and testicular Leydig cell activity, it plays an important role in assessing overall endocrine homeostasis, particularly within the hypothalamic-pituitary-gonadal and adrenal axes.
In clinical settings, androstenedione measurement is most commonly used in the evaluation of hyperandrogenism, including polycystic ovary syndrome (PCOS), congenital adrenal hyperplasia (CAH—especially 21-hydroxylase deficiency), adrenal tumors, and certain ovarian neoplasms. Elevated levels can help identify androgen excess of adrenal versus ovarian origin when combined with other markers such as DHEA-S, testosterone, and 17-hydroxyprogesterone. In pediatric populations, androstenedione testing supports the assessment of precocious puberty or disorders of sexual development. It can also be used to monitor therapeutic response in patients receiving glucocorticoid treatment for CAH or other endocrine disorders.
In research environments, androstenedione serves as a valuable biomarker for studying steroidogenesis, endocrine-disrupting chemicals, metabolic disorders, and reproductive physiology. Its role as a precursor to both androgens and estrogens makes it a key analyte in studies exploring sex hormone balance, fertility, and stress-related endocrine responses. Androstenedione assays are also applied in translational and preclinical research examining adrenal function, mechanisms of androgen excess, and the impact of pharmaceuticals on steroid hormone pathways.

This product is manufactured in USA by Eagle Biosciences.