1-Methylhistamine ELISA Kit

$670.00

The 1-Methylhistamine ELISA kit is an enzyme-linked immunosorbent assay used for the quantitative and very sensitive determination of 1-Methylhistamine in human urine samples. The 1-Methylhistamine ELISA kit is for research use only and should not be used in diagnostic procedures.

SKU: EA208/96 Category: Tags: , , , ,

1-Methylhistamine is a primary urinary metabolite of histamine, a biogenic amine synthesized from histidine by the enzyme histidine decarboxylase. Histamine plays a central role in immune responses, particularly in immediate allergic reactions where mast cells and basophilic granulocytes release it in response to allergen binding to IgE antibodies. Once released, histamine is rapidly inactivated in the body through enzymatic conversion by histamine-N-methyltransferase, producing 1-methylhistamine. This metabolite is water-soluble and excreted in urine, making it a useful biomarker for evaluating histamine turnover and mast cell activity.

In research and clinical settings, urinary 1-methylhistamine serves as a non-invasive biomarker for monitoring allergic conditions, mast cell disorders, and histamine-related pathophysiology. Its quantification can aid in diagnosing conditions such as systemic mastocytosis, food allergies, and other hypersensitivity reactions. Unlike histamine, which is unstable in circulation, 1-methylhistamine provides a more reliable indicator of histamine activity over time due to its stability and accumulation in urine.

The 1-Methylhistamine ELISA is a competitive enzyme immunoassay designed for the quantitative determination of this metabolite in human urine samples. The assay involves derivatization of 1-methylhistamine to N-acyl-1-methylhistamine, followed by competitive binding to a solid-phase antibody and detection through a peroxidase-TMB reaction measured at 450 nm. The inverse relationship between signal and analyte concentration enables sensitive detection of 1-methylhistamine levels, supporting both diagnostic and investigational research into allergic and immunologic responses.

This product is manufactured in Germany by DLD Diagnostika.

Size 1 x 96 Well
Sensitivity 3.0 ng/mL
Dynamic Range 10 – 1000 ng/mL
Incubation Time 1.5 hours
Sample Type Urine
Immunogen
Source Species
Specificity
Expression System
Clone
Group
Alpha Chain
Beta Chain
Peptide
Peptide Source
Format
Buffer
Concentration
Storage 2-8°C
Alternative Names 1-methylhistamine, N-methylhistamine, methylated histamine metabolite, histamine-N-methyltransferase product, monomethylhistamine, urinary histamine metabolite, methylhistamine derivative, histamine breakdown product, histamine catabolite, imidazole ethylamine methyl analog
Instructions For Use https://eaglebio.com/wp-content/uploads/2025/05/RUO-EA208-96-1-Methylhistamine-ELISA-Kit-IFU.pdf
MSDS https://eaglebio.com/wp-content/uploads/2014/06/MHU34-K01-1-Methylhistamine-ELISA-Assay-Kit-MSDS.pdf

Product Citations