Testosterone Total ELISA Kit

Testosterone is the primary male sex hormone, playing a crucial role in the development of male reproductive organs, secondary sexual characteristics such as facial hair and muscle mass, and overall male physiology. In men, testosterone levels reflect the functional capacity of Leydig cells in the testes and are commonly used as a biomarker for diagnosing hypogonadism (low levels) or hypergonadism (elevated levels). In women, testosterone is produced in smaller amounts by the adrenal glands and ovaries, and elevated levels can indicate underlying conditions such as polycystic ovary syndrome (PCOS), Cushing’s syndrome, enzyme deficiencies, or androgen-secreting tumors.

In clinical and research settings, total testosterone measurement is essential for evaluating reproductive health, endocrine disorders, and gender-related hormone therapy. It is also used in assessing infertility, monitoring hormone replacement therapy, and investigating conditions like delayed puberty in males or hirsutism and virilization in females. Testosterone is typically measured in serum or plasma using sensitive assays that can detect physiological and pathological concentrations accurately.

The Testosterone Total ELISA assay is a competitive immunoassay designed for the quantitative determination of testosterone in biological samples. In this format, testosterone in the sample competes with an enzyme-labeled testosterone conjugate for binding to limited antibody sites on the microplate wells. After a wash step to remove unbound components, the HRP-TMB colorimetric reaction produces a measurable signal inversely proportional to testosterone concentration. Absorbance is read at 450 nm, and values are calculated using a standard curve, allowing for precise hormone profiling in both clinical diagnostics and hormone-related research studies.

This product is manufactured in USA by Eagle Biosciences.