Thiobarbituric acid reactive substances (TBARS) are byproducts of lipid peroxidation, formed when reactive oxygen species (ROS) attack polyunsaturated fatty acids in cell membranes. Malondialdehyde (MDA) is the most commonly measured TBARS molecule and serves as a widely recognized biomarker of oxidative stress. Elevated TBARS levels indicate increased lipid peroxidation, reflecting cellular damage associated with aging, inflammation, and various pathological conditions, including cardiovascular disease, neurodegenerative disorders, and metabolic syndrome.
The TBARS ELISA (enzyme-linked immunosorbent assay) allows for the sensitive and specific quantification of TBARS in biological samples such as plasma, serum, urine, or tissue extracts. In research settings, TBARS ELISAs are used to study oxidative stress mechanisms, evaluate the efficacy of antioxidants, and investigate the role of lipid peroxidation in disease models. This assay provides valuable insights into cellular responses to oxidative damage and helps correlate oxidative stress with disease progression or therapeutic interventions.
In clinical applications, TBARS measurements can assist in monitoring patients with conditions linked to oxidative stress, such as diabetes, atherosclerosis, liver disease, and neurodegeneration. The assay is useful for evaluating the impact of lifestyle modifications, pharmacological treatments, or dietary interventions aimed at reducing oxidative damage. By providing a quantitative assessment of lipid peroxidation, TBARS ELISAs help clinicians and researchers better understand the oxidative component of disease processes and treatment outcomes.
This product is manufactured in by Eagle Biosciences.
| Size | 1 x 96 Well |
| Sensitivity | 0.5 µM |
| Dynamic Range | 0.5 – 20 µM |
| Incubation Time | 30 minutes |
| Sample Type | Serum, Plasma, Urine |
| Alternative Names | Thiobarbituric Acid Reactive Substances, Malondialdehyde Assay, and Lipid Peroxidation Assay |
