Neutrophil gelatinase-associated lipocalin (NGAL), also known as lipocalin-2, is a small glycoprotein expressed in neutrophils and various epithelial tissues. It plays a key role in innate immunity by binding and sequestering iron-containing siderophores, limiting bacterial growth. NGAL is also rapidly upregulated in response to cellular stress, injury, or inflammation, particularly in the kidney, making it a sensitive biomarker for tissue damage. In rodents, including rats, NGAL expression increases markedly during acute kidney injury (AKI) and other inflammatory conditions, reflecting its dual role in immune defense and tissue injury response.
The rat NGAL ELISA is an immunoassay designed to quantitatively measure NGAL levels in serum, plasma, urine, or tissue homogenates. In research settings, this assay is widely used to study kidney injury models, inflammatory responses, and metabolic disorders, providing a sensitive and early indicator of tissue stress before conventional markers like serum creatinine change. It is also employed in studies of bacterial infections, cancer, and cardiovascular disease, where NGAL modulation is of interest.
In a translational or preclinical clinical context, rat NGAL ELISAs help evaluate the efficacy and safety of therapeutic interventions targeting kidney protection, inflammation, or infection. By providing a reliable measure of early tissue injury or inflammatory response, NGAL monitoring can guide dosing, assess drug toxicity, and support mechanistic studies of disease progression. Its sensitivity and rapid response make NGAL a valuable biomarker for both experimental research and preclinical evaluation of potential clinical therapies.
This product is manufactured in USA by Eagle Biosciences.
| Size | 1 x 96 Well |
| Dynamic Range | 0.25 ng/mL – 8 ng/mL |
| Incubation Time | 1 hour 50 minutes |
| Sample Type | Serum, plasma, urine |
| Storage | 2-8°C |
| Alternative Names | Lipocalin-2, Neutrophil Gelatinase-Associated Lipocalin, Siderocalin, and 24p3. |