Nitrotyrosine ELISA Kit

Nitrotyrosine is a biomarker formed when tyrosine residues on proteins undergo nitration, typically driven by reactive nitrogen species such as peroxynitrite. This modification occurs during periods of oxidative and nitrosative stress, where reactive oxygen and nitrogen intermediates interact to damage lipids, proteins, and nucleic acids. Because nitrotyrosine is relatively stable and accumulates on proteins, it serves as a footprint of nitric oxide–derived oxidative reactions and is widely used to assess cellular or tissue-level oxidative injury.
In research settings, nitrotyrosine measurement is commonly used to study mechanisms of inflammation, mitochondrial dysfunction, cardiovascular disease, neurodegeneration, and metabolic disorders. Techniques such as ELISA, Western blotting, mass spectrometry, and immunohistochemistry allow investigators to quantify protein nitration and localize oxidative damage within tissues. Nitrotyrosine levels help researchers understand pathways involving nitric oxide synthase dysregulation, oxidative signaling cascades, and the impact of pharmacological interventions designed to reduce oxidative stress.
Clinically, nitrotyrosine is considered an emerging biomarker rather than a routine diagnostic test, but it has shown significant potential in several disease areas. Elevated nitrotyrosine has been associated with conditions such as atherosclerosis, diabetes, chronic kidney disease, sepsis, and various neurologic disorders, reflecting heightened oxidative stress. It is being explored for risk stratification, prognosis, and monitoring therapeutic response in diseases where oxidative injury contributes to pathology. Although not yet standardized for widespread clinical use, nitrotyrosine remains a valuable translational biomarker linking mechanistic oxidative stress research to potential clinical applications.

This product is manufactured in Canada by StressMarq.