Mouse Immunoglobulin G (IgG) is the most abundant antibody isotype in mouse serum and serves as a key component of the adaptive immune response. It consists of two heavy and two light chains, forming a Y-shaped structure responsible for antigen binding and immune effector functions such as opsonization, complement activation, and neutralization. The Mouse IgG ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used immunoassay designed to quantitatively measure total IgG or antigen-specific IgG antibodies in mouse samples such as serum, plasma, or cell culture supernatants. The assay typically employs an anti-mouse IgG capture antibody and an enzyme-conjugated secondary antibody to produce a colorimetric signal proportional to IgG concentration.
In research, Mouse IgG ELISA is fundamental for evaluating immune responses in vaccination, infection, and autoimmune disease models. It allows precise measurement of antibody titers following immunization or exposure to pathogens, making it a cornerstone of immunology and vaccine efficacy studies. Researchers also use it to monitor monoclonal antibody production in hybridoma or recombinant expression systems and to assess humoral immunity in genetically modified or immunocompromised mice. The assay’s quantitative nature provides a reliable metric for comparing immune responses across experimental groups or time points.
While the Mouse IgG ELISA is primarily a research tool, its applications have strong translational implications. Mouse models are extensively used to predict human antibody responses and guide the development of therapeutic antibodies and vaccines. Quantifying IgG levels helps researchers understand mechanisms of immune protection, autoimmunity, or immunopathology, which informs preclinical safety and efficacy assessments. In summary, the Mouse IgG ELISA is a quantitative biomarker assay central to immunological research, enabling precise monitoring of antibody-mediated responses that translate directly to diagnostic and therapeutic innovations in human medicine.
This product is manufactured in USA by Eagle Biosciences.