Matrix metalloproteinase-19 (MMP-19) is a member of the MMP family of zinc-dependent endopeptidases that remodel the extracellular matrix (ECM). Unlike many other MMPs that are only expressed in response to injury or disease, MMP-19 is constitutively expressed in many tissues, including keratinocytes, endothelial cells, and fibroblasts. ([PubMed][1]) Functionally, MMP-19 can degrade a variety of ECM components (e.g., type IV collagen, fibronectin, nidogen) and has both proteolytic and non-proteolytic roles, influencing cell migration, proliferation, and tissue remodeling. ([Paginas Personales UNAM][2])
More intriguingly, MMP-19 has been implicated in both **pro-angiogenic** and **anti-angiogenic** processes. For example, it can generate angiostatin-like fragments from plasminogen, which inhibit endothelial proliferation and capillary formation, suggesting a regulatory role in vascular remodeling. ([PubMed][3]) In disease contexts, dysregulation of MMP-19 has been linked to cancer (e.g., higher expression in non-small cell lung cancer correlates with invasiveness and poor prognosis) ([PMC][4]), as well as fibrosis: in idiopathic pulmonary fibrosis (IPF), MMP-19 appears to protect against excessive fibrotic progression by promoting epithelial wound healing and inducing prostaglandin-related pathways. ([PMC][5])
In animal models, genetic deletion of MMP-19 has yielded important insights: MMP-19–deficient mice exhibit worsened lung fibrosis in response to bleomycin, highlighting its reparative role, ([PMC][5]) but also display altered immune responses (e.g., in colitis, MMP-19 loss leads to worse inflammation due to impaired macrophage migration and chemokine processing). ([PubMed][6])
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### Uses of the Hamster CHO MMP-19 ELISA Kit in Research & Potential Clinical Context
The **[Hamster CHO MMP‑19 ELISA Kit](https://www.abcam.com/en-us/products/elisa-kits/hamster-cho-mmp-19-elisa-kit-ab313942?utm_source=chatgpt.com)** is a sandwich ELISA designed to quantitatively detect MMP-19 in culture supernatants derived from Chinese hamster ovary (CHO) cells. The assay has a sensitivity range of 0.3125–20 ng/mL, making it suitable for measuring secreted MMP-19 in in vitro systems. ([Abcam][7]) This is particularly useful in **research settings** such as:
1. **Cell biology / mechanistic studies**: You can use the kit to monitor how various treatments (e.g., growth factors, cytokines, drugs) regulate MMP-19 secretion from CHO cells or other engineered cell lines expressing hamster MMP-19.
2. **Drug screening**: Since MMP-19 is implicated in pathological remodeling (e.g., fibrosis, cancer), the kit allows screening of small molecules or biologics that modulate its expression or secretion.
3. **Recombinant protein production**: If you’re producing hamster MMP-19 recombinantly (e.g., for structural studies), this ELISA can serve as a quality control tool to quantify how much active protein is secreted into the medium.
In a **clinical setting**, direct applications of this kit are limited because:
* **Species specificity**: It’s designed for the hamster (CHO) version of MMP-19, so it is not directly translatable to human clinical diagnostics.
* **Research-only designation**: According to the manufacturer, the kit is for **research use only**, not for diagnostic purposes. ([Abcam][8])
However, the biological insights gained from hamster or other animal models using this ELISA could **indirectly inform clinical research**. For example, findings on how MMP-19 is regulated in fibrosis or inflammatory disease models might guide biomarker development in human disease. It could also facilitate **preclinical testing** of therapies targeting MMP-19 signaling pathways before translation to human trials.
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If you like, I can also summarize known cross-species conservation (e.g., how similar hamster MMP-19 is to human) to help you interpret your data — do you want me to do that?
[1]: https://pubmed.ncbi.nlm.nih.gov/14708597/?utm_source=chatgpt.com “Matrix metalloproteinase-19 expression in normal and diseased skin: dysregulation by epidermal proliferation – PubMed”
[2]: https://www.paginaspersonales.unam.mx/files/4263/Publica_20160107194133.pdf?utm_source=chatgpt.com “Matrix Metalloproteinase-19 Is a Key Regulator of Lung”
[3]: https://pubmed.ncbi.nlm.nih.gov/21787393/?utm_source=chatgpt.com “Matrix metalloproteinase-19 inhibits growth of endothelial cells by generating angiostatin-like fragments from plasminogen – PubMed”
[4]: https://pmc.ncbi.nlm.nih.gov/articles/PMC4299607/?utm_source=chatgpt.com “Matrix Metalloproteinase-19 Promotes Metastatic Behavior In Vitro and Is Associated with Increased Mortality in Non–Small Cell Lung Cancer – PMC”
[5]: https://pmc.ncbi.nlm.nih.gov/articles/PMC5450991/?utm_source=chatgpt.com “Matrix Metalloproteinase-19 Is a Key Regulator of Lung Fibrosis in Mice and Humans – PMC”
[6]: https://pubmed.ncbi.nlm.nih.gov/26555704/?utm_source=chatgpt.com “MMP-19 deficiency causes aggravation of colitis due to defects in innate immune cell function – PubMed”
[7]: https://www.abcam.com/en-us/products/elisa-kits/hamster-cho-mmp-19-elisa-kit-ab313942?utm_source=chatgpt.com “Hamster CHO MMP-19 ELISA Kit (ab313942) | Abcam”
[8]: https://www.abcam.cn/products/elisa-kits/hamster-cho-mmp-19-elisa-kit-ab313942.html?utm_source=chatgpt.com “仓鼠CHO MMP-19 ELISA试剂盒 (ab313942)| Abcam中文官网”
This product is manufactured in USA by Eagle Biosciences.
| Size | 1 x 96 Well |
| Sensitivity | 0.054 ng/mL |
| Dynamic Range | 20 ng/mL – 0.3125 ng/mL |
| Incubation Time | 2 hours 50 minutes |
| Sample Type | Tissue Culture Fluids |
| Storage | 2-8°C |
| Alternative Names | 1. This kit is also known as the Hamster CHO MMP-19 Quantitative ELISA Kit. 2. This kit is also referred to as the Hamster CHO MMP19 Immunoassay Kit. 3. This kit is also called the Hamster CHO Matrix Metalloproteinase-19 Detection Kit. 4. This kit is likewise known as the Hamster CHO MMP-19 Sandwich ELISA Kit. 5. This kit may also be described as the Hamster CHO MMP-19 Protein ELISA Kit. 6. This kit is alternatively termed the Hamster CHO MMP-19 Antigen Assay Kit. 7. This kit is also labeled as the Hamster CHO MMP-19 Enzyme Immunoassay Kit. |