Hampster (CHO) PLBL2 ELISA

$1,510.00

The Eagle Biosciences Hamster (CHO) PLBL2 ELISA Assay Kit is a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring PLBL2 as a host cell impurity protein in cultures derived from Chinese hamster ovary cells.

SKU: HPB93-K01 Category: Tags: , , ,

Phospholipase B-like 2 (PLBL2) is a lysosomal enzyme that can be secreted by Chinese Hamster Ovary (CHO) cells, the predominant cell line used for manufacturing therapeutic monoclonal antibodies and other recombinant biologics. Although not typically a focus in basic biological research, PLBL2 has gained attention in biopharmaceutical development as a host cell protein (HCP) impurity. During cell culture and downstream processing, PLBL2 may co-purify with therapeutic proteins, particularly monoclonal antibodies, and persist in the final drug product if not sufficiently cleared.

In research and clinical settings, PLBL2 is monitored primarily as a critical quality attribute due to its potential to trigger immune responses in patients. Studies have shown that residual PLBL2 can act as an immunogenic impurity, stimulating the production of anti-PLBL2 antibodies and potentially interfering with therapeutic efficacy or causing adverse immune effects. Consequently, sensitive immunoassays, such as ELISAs or mass spectrometry-based methods, are developed to detect and quantify PLBL2 during process validation, lot release, and immunogenicity risk assessments. Understanding and controlling PLBL2 levels is essential in ensuring the safety, purity, and regulatory compliance of CHO-derived biologics.

This product is manufactured in USA by Eagle Biosciences.

Size 1 x 96 Well
Sensitivity
Dynamic Range 20 ng/mL – 0.3125 ng/ml
Incubation Time 170 minutes
Sample Type Tissue Culture Fluids
Immunogen
Source Species
Specificity
Expression System
Clone
Group
Alpha Chain
Beta Chain
Peptide
Peptide Source
Format
Buffer
Concentration
Storage 2-8°C
Alternative Names PLBL2, Endogenous CHO PLBL2, Host Cell Protein PLBL2
Instructions For Use https://eaglebio.com/wp-content/uploads/2019/04/RUO_CGE41-K01-Canine-IgE_ELISA_Assay_Kit_Package_Insert-2.pdf
MSDS https://eaglebio.com/wp-content/uploads/2019/04/HPB93-K01-Hamster-PLBL2-ELISA-Assay-MSDS.pdf

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