easYmer HLA-A*30:02 MHC Tetramers Kit

$390.00$5,490.00

easYmer® HLA-A*30:02 MHC Tetramers Kit can be used to generate monomers with your choice of peptide. The monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to analyze T-cells by flow cytometry. The easYmer reagent can also be used to evaluate specific peptide-HLA I binding.

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easYmer HLA-A*30:02 MHC Tetramers Kit

for Research Use Only

Materials Included:

  • easYmer: HLA-A*30:02 – peptide receptive and biotinylated HLA-A*30:02 molecules in Tris/Maleate pH7 with 30% Glycerol
  • Folding Buffer: Tris/Maleate pH7
  • Peptide: YF NS5 286-295 (KSEYMTSWFY), a known HLA-A*30:02 binder. Positive control for evaluation analysis of peptide-HLA folding

Available Sizes:

    • 20 Tests (Sample-size)
    • 50 Tests (Standard Size)
    • 150 Tests
    • 500 Tests

    Key Benefits of easYmer® MHC Tetramers

    • Ready-to-use
    • One step loading
    • Completely flexible and customizable*
    • Biotinylated
    • No special equipment needed
    • Long shelf-life

    *For Custom Tetramer Production, please Contact Us for more information


    Product Developed and Manufactured by immunAware

Additional Information

Assay Background


The easYmer® MHC Tetramers Kit features a highly active formulation of HLA class I (HLA-I) molecules, which can be used to generate specific peptide-HLA class I monomers of your choice in your own laboratory. These monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to stain antigen specific T-cells for analysis in flow cytometric assays. Optionally, the monomers can be stored frozen for later use. The easYmer® technology is highly flexible and suitable for screening of a single epitope in a large number of samples, as well as for screening of a large number of different epitopes in parallel.

Assay Principle


This protocol is designed to evaluate the efficiency of peptide-HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain subunit of recombinant HLA Class I (HLA-I) comlexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide-HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.

Manual

Product Manual


Publications

Citations


  • Abana, C.O., et al., Cytomegalovirus (CMV) Epitope-Specific CD4(+) T Cells Are Inflated in HIV(+) CMV(+) Subjects. J Immunol, 2017. 199(9): p. 3187-3201.
  • Nastasi, C., et al., Butyrate and propionate inhibit antigen-specific CD8(+) T cell activation by suppressing IL-12 production by antigen-presenting cells. Sci Rep, 2017. 7(1): p. 14516.
  • Gonzalez-Duque, S., et al., Conventional and Neo-antigenic Peptides Presented by beta Cells Are Targeted by Circulating Naive CD8+ T Cells in Type 1 Diabetic and Healthy Donors. Cell Metab, 2018. 28(6): p. 946-960 e6.
  • Moyo, N., et al., Efficient Induction of T Cells against Conserved HIV-1 Regions by Mosaic Vaccines Delivered as Self-Amplifying mRNA. Mol Ther Methods Clin Dev, 2019. 12: p. 32-46.