easYmer HLA-A*01:01 MHC Tetramers Kit

$1,050.00$2,425.00

easYmer® HLA-A*01:01 MHC Tetramers Kit can be used to generate monomers with your choice of peptide. The monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to analyze T-cells by flow cytometry. The easYmer reagent can also be used to evaluate specific peptide-HLA I binding. easYmer® HLA-A*01:01 MHC Tetramers Kit is for research use only.

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easYmer HLA-A*01:01 MHC Tetramers Kit

easYmer HLA-A*01:01 MHC Tetramers Developed and Manufactured by immunAware

Materials Included:

The easYmer kit contains a peptide receptive preparation of HLA, a folding buffer, a positive control peptide for HLA-complex folding. The exact identities of these components are given below.

  • easYmer: HLA-A*01:01 – peptide receptive and biotinylated HLA-A*01:01 molecules in Tris/Maleate pH7 with 30% Glycerol
  • Folding Buffer: Tris/Maleate pH7
  • Peptide: YF NS5 286-295 (KSEYMTSWFY), a known HLA-A*01:01 binder. Positive control for evaluation analysis of peptide-HLA folding

Available Sizes:

  • 50 Tests (Standard Size)
  • 150 Tests
  • Contact Us for Bulk orders

Key Benefits of easYmer® MHC Tetramers

  • Ready-to-use
  • One step loading
  • Completely flexible and customizable*
  • Biotinylated
  • No special equipment needed
  • Long shelf-life

*For Custom Tetramer Production, please Contact Us for more information

For Research Use Only
Eagle Biosciences, Inc is an authorized agent for ImmunAware


Assay Principle


This protocol is designed to evaluate the efficiency of peptide HLA-I interaction and complex formation. The assay is based on detecting the ß2-microglobulin (ß2m? light chain sub unit of recombinant HLA Class I (HLA-I) complexes, where the heavy chain has been biotin tagged. These tagged complexes are subsequently captured by streptavidin coated beads, labeled with PE-conjugated anti-human ß2m, and analyzed by flow cytometry. Since peptide HLA-I complex formation is entirely peptide dependent, bead-associated signals will only be detected if the peptide in question supports the folding of the HLA-I allotype of interest; peptides that efficiently support folding will give strong signals whereas peptides that support folding sub-optimally, or not at all, will give moderate to non-detectable signals.


Related Products

easYmer HLA-A*02:01 MHC Tetramers Kit
easYmer HLA-A*02:03 MHC Tetramers Kit
easYmer HLA-A*03:01 MHC Tetramers Kit

Additional Information

Assay Background


The easYmer® MHC Tetramers Kit features a highly active formulation of HLA class I (HLA-I) molecules, which can be used to generate specific peptide-HLA class I monomers of your choice in your own laboratory. These monomers can easily be tetramerized with fluorophore conjugated streptavidin and used to stain antigen specific T-cells for analysis in flow cytometric assays. Optionally, the monomers can be stored frozen for later use. The easYmer® technology is highly flexible and suitable for screening of a single epitope in a large number of samples, as well as for screening of a large number of different epitopes in parallel.

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Citations


  • Abana, C.O., et al., Cytomegalovirus (CMV) Epitope-Specific CD4(+) T Cells Are Inflated in HIV(+) CMV(+) Subjects. J Immunol, 2017. 199(9): p. 3187-3201.
  • Nastasi, C., et al., Butyrate and propionate inhibit antigen-specific CD8(+) T cell activation by suppressing IL-12 production by antigen-presenting cells. Sci Rep, 2017. 7(1): p. 14516.
  • Gonzalez-Duque, S., et al., Conventional and Neo-antigenic Peptides Presented by beta Cells Are Targeted by Circulating Naive CD8+ T Cells in Type 1 Diabetic and Healthy Donors. Cell Metab, 2018. 28(6): p. 946-960 e6.
  • Moyo, N., et al., Efficient Induction of T Cells against Conserved HIV-1 Regions by Mosaic Vaccines Delivered as Self-Amplifying mRNA. Mol Ther Methods Clin Dev, 2019. 12: p. 32-46.

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