DPYD HT qPCR Kit
The APIS DPYD HT qPCR Kit is designed for a multiplexed, High Throughput (HT), fast and efficient detection of seven DPYD alleles. Each reaction identifies both wildtype (WT) and variant alleles, directly from blood or extracted genomic DNA (gDNA). The kit’s rapid turnaround time, cost-effectiveness, and easy integration into existing qPCR workflows make it a reliable and accessible option for a variety of applications.
Advantages of the APIS DPYD HT qPCR Kit
– Wide target coverage – detects seven DPYD variants
– Direct Workflow – compatible with both 1% EDTA Whole Blood and gDNA
– Reliable HapB3 detection – targets key functional HapB3 variant
– High throughput – 4 reactions per sample supports efficient testing
The analytical sensitivity of the kit was evaluated by testing homozygous (wildtype) and heterozygous samples and defined as the minimum amount of DNA necessary to correctly genotype 95% of the samples. The APIS DPYD HT qPCR Kit can detect seven DPYD variants in as little as 0.11ng gDNA.
Compatible with most real-time PCR instruments and can be implemented easily into your current PCR workflows. Provides testing throughput flexibility as 22 samples + controls can be run on a 96-well plate, or supports low throughput testing with enough reagents for up 8x PCR runs. Negative controls, positive controls and reference DPYD WT target amplification provide QC for sample input and PCR amplification.
APIS DPYD HT qPCR Kit shows excellent concordance with alternative research genotyping methods. gDNA extracted from 29 specimens with known DPYD variant status (characterised via NGS, microarrays or qPCR) and one synthetic DNA sample were assessed with the APIS DPYD qPCR Kit.
DPYD Variant (NM_000110.4) and rsID
c.1905+1G>A rs3918290
c.1679T>G rs55886062
c.1129-5923C>G rs75017182
c.557A>G rs115232898
c.2846A>T rs67376798
c.868A>G rs146356975
c.2279C>T rs112766203
Kit size: 24 samples plus controls, includes: 4x Primer Probe mixes, 2x Enzyme Mixes, Positive Control, No Template Control (NTC).
The APIS DPYD HT qPCR Kit is available as a Research Use Only (RUO) product. Not applicable for use in diagnostic procedures.
This DPYD HT qPCR Kit is manufactured in United Kingdom by APIS Assay Technologies.
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