Dopamine ELISA Kit

$595.00

The Dopamine ELISA Kit is intended for the quantitative determination of Dopamine in urine or plasma. The Dopamine ELISA Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

SKU: EA608/96 Category: Tags: , , ,

Dopamine is a vital catecholamine neurotransmitter and precursor to both adrenaline and noradrenaline. Synthesized in the brain and peripheral nervous system, dopamine plays a key role in regulating movement, motivation, reward behavior, and neuroendocrine signaling. It also participates in the body’s stress response, influencing cardiovascular and metabolic functions indirectly through its conversion to other catecholamines. As a biochemical messenger, dopamine is critical to neurological health and is often studied in the context of neurodegenerative and psychiatric conditions.

In clinical and research applications, dopamine serves as a valuable biomarker for diagnosing and monitoring various disorders. Abnormal dopamine levels are associated with hypertension, cardiac dysfunction, schizophrenia, and bipolar disorder. Importantly, dopamine and its metabolites are of high diagnostic value in pediatric populations, particularly for detecting neuroblastoma—a tumor of the sympathetic nervous system. Measurement of dopamine levels in plasma or urine helps clinicians evaluate catecholaminergic activity and identify underlying pathologies related to autonomic nervous system function or neurochemical imbalance.

The Dopamine ELISA kit enables sensitive and specific quantification of dopamine in biological samples such as plasma and urine. Dopamine is first extracted using a cis-diol-specific affinity gel, acylated into N-acyl-dopamine, and then enzymatically converted into N-acyl-3-methoxytyramine. This competitive immunoassay format involves competition between sample-derived and solid-phase dopamine for antibody binding. Detection is achieved via a TMB-peroxidase colorimetric reaction, read at 450 nm, where signal intensity is inversely proportional to dopamine concentration. This assay is a powerful tool for advancing neurochemical research and supporting clinical diagnostics in dopamine-related diseases.

This product is manufactured in Germany by DLD Diagnostika.

Size 1 x 96 Well
Sensitivity Urine: 0.43 ng/mL;
EDTA Plasma: 29 pg/mL
Dynamic Range 1.5 – 2560 ng/mL
Incubation Time Overnight
Sample Type Urine, EDTA-Plasma
Immunogen
Source Species
Specificity
Expression System
Clone
Group
Alpha Chain
Beta Chain
Peptide
Peptide Source
Format
Buffer
Concentration
Storage 2-8°C
Alternative Names Dopamine, 3-hydroxytyramine, DA, catecholamine neurotransmitter, N-acyl-dopamine, motor control modulator, reward pathway messenger, neuroendocrine regulator, dopamine precursor compound, N-acyl-3-methoxytyramine
Instructions For Use https://eaglebio.com/wp-content/uploads/2025/05/RUO-EA608-96-Dopamine-ELISA-Kit-IFU.pdf
MSDS https://eaglebio.com/wp-content/uploads/2014/06/CAT-ELISA-Assay-Kits-MSDS.pdf

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