Adrenaline, also known as epinephrine, is a catecholamine hormone and neurotransmitter derived from dopamine and plays a central role in the body’s acute stress response. Produced primarily in the adrenal medulla, adrenaline acts on cardiac musculature and metabolic processes to increase heart rate, boost energy availability, and prepare the body for “fight-or-flight” reactions. It is essential for maintaining cardiovascular stability during times of physical or emotional stress and serves as a key mediator in sympathetic nervous system activation.
In clinical and research settings, adrenaline is an important biomarker for evaluating stress-related disorders and diseases involving catecholamine dysregulation. Abnormal levels may be associated with chromaffin cell tumors such as pheochromocytoma, neuroblastoma, or ganglioneuroma, and can also indicate conditions such as hypertension, heart disease, schizophrenia, or bipolar disorder. Measuring adrenaline concentrations in plasma or urine helps assess autonomic nervous system activity, diagnose catecholamine-producing tumors, and monitor treatment responses in both psychiatric and cardiovascular conditions.
The Adrenaline ELISA kit provides a reliable method for quantifying adrenaline levels in biological samples. The assay involves extraction using a cis-diol-specific affinity gel, derivatization to N-acyladrenaline, and enzymatic conversion into N-acylmetanephrine. In this competitive immunoassay, acylated adrenaline from the sample competes with plate-bound adrenaline for antibody binding sites. The signal, developed through a TMB-peroxidase reaction and measured at 450 nm, is inversely proportional to the sample’s adrenaline concentration. This assay enables precise, sensitive detection of adrenaline, supporting both diagnostic workflows and research into stress-related biochemical pathways.
This product is manufactured in Germany by DLD Diagnostika.