At this time it is unknown for how long antibodies persist following infection or vaccination, and if the presence of antibodies confers protective immunity. Antibodies to SARS-CoV-2 are generally detectable in blood several days after initial infection or vaccination, although the duration of time antibodies are present post-infection or post-vaccination is not well characterized. Individuals may have detectable virus present for several weeks following seroconversion.
The Cov19 FluoBolt-DAT test, manufactured in Austria by Fianostics GmbH, is a duplex antibody test that is intended to identify individuals with an adaptive immune response to SARS-CoV-2, indicating recent or prior infection or vaccination. There is a high demand to know how protected populations are after COVID-19 onset or vaccination, this test is the next step in identifying immunity.
How the Cov19 FluoBolt-DAT Test Works
When performing the assay, 10 µl of a patient serum or plasma sample are simultaneously mixed with 50 µl of two different fluorescence-labeled tracer antibodies against the S1RBD and against the NC protein and incubated in our special fluorescence-enhancing microtiter plate for 60 minutes. By measuring at 2 different wavelengths, their displacement by homologous antibodies from the patient sample is determined simultaneously. The signals at one wavelength provide information about the concentration of anti-S1RBD antibodies. The signals at the other wavelength indicate the concentration of anti-nucleocapsid antibodies.
By using the supplied calibrators, a calibration curve for the anti-S1RBD and the antiNC antibodies is constructed, and the antibody concentration of a patient sample is read from it. With just a single measurement, this results in a quantitative determination of these
two antibody species against SARS-CoV-2, which represent the majority of the immune response and may protect against infection and illness.
For measurement, any commercially available fluorescence microtiter plate reader can be used.
Click here to learn more about the Cov19 FluoBolt-DAT test.
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