Histamine ELISA Assay Kit
Histamine ELISA Assay Kit manufactured in Germany as SKU EA213/96 by DLD Diagnostika
Size: 1×96 wells
Sensitivity: 0.06 ng/ml (plasma), 0.9 ng/ml (urine)
Dynamic Range: 0.15 – 25 ng/ml
Incubation Time: 2.5 hours
Sample Type: Plasma (EDTA or Heparin), Whole Blood, Urine and Cell Culture
Sample Size: 50 µl
For Research Use Only
Reference Values (Normal)
Normal range EDTA plasma:< 1 ng/ml
Normal Range Heparin Plasma: <5.4 ng/ml
Normal Range Heparin Whole Blood: 10-100 ng/ml
Normal range urine: < 45 µg/day
It is suggested that each laboratory establish its own normal ranges for the Histamine ELISA
Conversion: 1 ng/ml corresponds to 9.0 nmol/l
Assay Background
Histamine (β-imidazole-ethylamine), a biogenic amine, is a product of the histidine metabolism. It is produced by decarboxylation of histidine. Histamine is widely distributed in mammalian tissues. It’s bound to heparin (as inactive form) and stored in the granules of basophilic leukocytes and mast cells and is actively released as required. These cells, if sensitized by IgE antibodies attached to their membranes, degranulate when exposed to the appropriate antigen.
Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.
Products Related to Histamine ELISA Assay
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Related News
Click the links below to learn more about studies using this ELISA
Histamine ELISA Assay Kit used in a recent study.
Boston Study: EagleBio’s Histamine ELISA