The **CHO LPLA2 (Lysosomal Phospholipase A2) ELISA Kit** is designed to measure levels of LPLA2, a lysosomal enzyme involved in phospholipid metabolism, membrane turnover, and cellular lipid homeostasis. LPLA2 plays an essential role in breaking down phospholipids within lysosomes and contributes to maintaining normal cell membrane composition. In Chinese Hamster Ovary (CHO) cells—widely used for biologics production—LPLA2 is a useful biomarker of lysosomal function, cellular stress, and metabolic activity. Altered LPLA2 expression or activity can indicate disruptions in lipid processing, lysosomal integrity, or cellular health.
In **research settings**, the CHO LPLA2 ELISA Kit is commonly used to assess how experimental conditions, genetic modifications, or bioprocessing variables affect CHO cell physiology. Researchers studying lipid metabolism, lysosomal biology, or stress pathways utilize LPLA2 measurement to monitor enzyme regulation and understand how cells respond to metabolic or environmental changes. It is also valuable for evaluating how culture conditions or therapeutic candidates influence lipid remodeling, lysosomal stability, and overall cell viability.
In **preclinical and biomanufacturing applications**, measuring LPLA2 helps optimize CHO-based production systems by identifying early signs of cellular dysfunction that could impact protein yield or quality. While LPLA2 quantification in CHO cells is not directly used in human clinical diagnostics, it supports therapeutic development by ensuring cell-line stability, improving product consistency, and helping predict potential lipid- or lysosome-related toxicities during drug development. The insights gained can guide better bioprocess design and facilitate translational studies where lysosomal dysfunction plays a role in human disease models.
This product is manufactured in USA by Eagle Biosciences.
| Size | 1 x 96 Well |
| Sensitivity | 0.138 ng/mL |
| Dynamic Range | 20 ng/mL – 0.3125 ng/mL |
| Incubation Time | 2 hours 50 minutes |
| Sample Type | Tissue culture fluids |
| Storage | 2-8°C |
| Alternative Names | Here are alternative names written **in sentence format**:
1. This assay is also known as a **CHO Lysosomal Phospholipase A2 ELISA Kit**. |