TBARS Fluorometric ELISA

Thiobarbituric Acid Reactive Substances (TBARS) are byproducts formed during the peroxidation of lipids, primarily polyunsaturated fatty acids, and serve as an indirect marker of oxidative stress. Malondialdehyde (MDA) is the most commonly measured TBARS compound and reflects the degree of lipid damage caused by reactive oxygen species (ROS). Elevated TBARS levels indicate oxidative damage to cellular membranes, which is associated with aging, inflammation, cardiovascular disease, neurodegeneration, and metabolic disorders. Measuring TBARS provides insight into the balance between oxidative stress and antioxidant defenses in biological systems.

The TBARS Fluorometric assay allows for sensitive and quantitative detection of TBARS in serum, plasma, tissue homogenates, and other biological samples. In research settings, it is widely used to study oxidative stress mechanisms, evaluate the impact of drugs or antioxidants, and investigate disease processes linked to lipid peroxidation. Researchers also employ TBARS measurements to assess cellular responses to environmental toxins, nutritional interventions, or experimental treatments that may modulate oxidative stress levels.

In clinical applications, TBARS assays can help monitor oxidative stress in patients with chronic diseases such as cardiovascular disorders, diabetes, liver disease, or neurodegenerative conditions. While not a diagnostic marker on its own, TBARS levels can complement other biomarkers to evaluate disease progression, therapeutic efficacy, or patient response to antioxidant therapies. Its simplicity, sensitivity, and adaptability make the TBARS fluorometric assay a valuable tool for both translational research and clinical investigations of oxidative damage.

This product is manufactured in by Eagle Biosciences.