Vitamin B1 Microtiter Plate Assay Kit

$890.00

The Eagle Biosciences Vitamin B1 is a microtiter plate test kit based on a microbiological assay which measures the vitamin B1 content in serum. The test kit contains all required reagents, e.g. standard, medium and microtiter plate coated with a specific microorganism, sufficient for 96 determinations including standard curves. An ELISA reader is required for evaluation of the vitamin B1 content.  The Vitamin B1 Assay kit is for research use only.

SKU: KIFR001 Categories: , ,

Vitamin B1 Microtiter Plate Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 3 µg/L
Dynamic Range: 3 – 15 µg/L
Incubation Time: 24 hours
Sample Type: Whole Blood
Sample Size: 100 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

The bioactive form of vitamin B1 is thiamine pyrophosphate. It plays an important role as a coenzyme in carbohydrate and amino acid metabolism. Thiamine pyrophosphate is a vital co-factor for enzymes involved in several key metabolic processes in the nervous system, the heart, the blood cells, and the muscle. Vitamin B1 assists in the conversion of carbohydrates into energy, necessary for healthy brain and nerve cells and heart function.

Vitamin B1 deficiency may result from a deficiency in the diet. Eventually, a severe vitamin B1 deficiency may lead to BERI-BERI, characterized by nerve, heart, and brain abnormalities. Deficiency may occur in alcoholics or in special clinical situations such as hemodialysis, chronic peritoneal dialysis, or after administration of glucose to a vitamin B1-depleted patient. Further vitamin B1 deficiency diseasesareWernicke‘sencephalopathy, Korsakow-syndrome, and some forms of Landry’s paralysis. Also, myopathy was found in relation to thiamine deficiency.

Assay Principle

The blood samples are enzymatically pre-treated and then transferred in the wells of a microtiter plate[PLATE] coated with Lactobacillusfermentum. The addition of vitamin B1 in either standard [STD], control [CTRL1, CTRL2] or samples gives a vitamin B1-dependent growth response until vitamin B1 is consumed. After incubation at 37 °C for 48 hours, the growth of Lactobacillus fermentum is measured turbidimetrically at 610 – 630 nm (alternatively at 540 – 550 nm) in an ELISA-reader and a standard curve is generated from the dilution series. The amount of vitamin B1 is directly proportional to the turbidity.

Manual

Product Manual