Troponin I ELISA Assay Kit


Eagle Biosciences Troponin I ELISA Assay Kit is an immunoenzymatic colorimetric method for quantitative determination of Troponin I concentration in human serum. Troponin I ELISA Assay Kit is intended for research use only and not intended for diagnostic procedures.

Troponin I ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 0.03 ng/ml
Dynamic Range: 1- 30 ng/ml
Incubation Time: 0.5 hour
Sample Type: Serum
Sample Size: 25 µL

Controls Included

Product Developed and Manufactured in Italy by Diametra

Additional Information

Assay Background

Troponin I is a 24kDa protein and is part of a complex, called Troponin complex, that regulates the calcium-modulate interaction between actin and myosin in the striated muscle; this complex is an heterodimer consisting of Troponin C, I and T; Troponin I is the inhibitory subunit of this complex.   Troponin complex is tightly bound to the contractile apparatus, but many studies shows that the Troponin I is released into the blood circulation in case of myocardial damage, such as in AMI (Acute Myocardial Infarction), where the myofibril is seriously damaged. The presence of Troponin I in the circulation can be detected for many days after AMI, thus the detection of Troponin levels in blood fluids is an excellent biomarker for myocardial damage.  It should be noted that in case of marathon runners or other skeletal injury, the Troponin I has not been found in circulation, thus it is a very specific marker for AMI.

Assay Principle

In the first step the serum sample and the Conjugate are added to the microplate coated with Streptavidin. The conjugate in this Troponin I ELISA Assay Kit contains two monoclonal antibodies direct against different epitopes of Troponin I: one is biotinylated, the other one is bound to the horseradish peroxidase (HRP); these antibodies compete for the native Troponin I in the sample, thus at the end of first step a sandwich between the antibodies and the Troponin I is formed; this complex binds to the microplate wells through the specific interaction between biotine and streptavidin.
At the end of incubation, the bound/free separation is performed by a simple solid-phase washing.  In the next step, the enzyme HRP in the bound-fraction reacts with the Substrate and develops a blue color; the reaction is stopped by adding the Stop Solution.  The concentration of Troponin I in the sample is calculated through a calibration curve.


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