T2/HT2 Immunoaffinity HPLC Column
The T2/HT2 Immunoaffinity HPLC Column is For Research Use Only
Size: 3ml, 10 columns
This instruction of T2/HT2 determination in food and feed focuses on the enrichment step of extract using immunoaffinity column (IAC) and quantification with HPLC.
Accepted laboratory extraction methods could be maintained. Full performance of the IAC column is given if pronounced criteria regarding organic solvent tolerance, elution process of analyte and working range of column is followed.
Many pretreatment methods for T2/HT2 in food and feed show low sensitivity because of interferrin substances if problematic matrices are applied.
This method of content determination of T2/HT2 combines the high selectivity of an immunoaffinity column (IAC with its potential to concentrate elute and additional step of purification of labelled T2/HT2 by HPLC column.
As said before, this instruction focuses on the handling with the IAC column. For the commodity extraction step a literature method is given. Please see below. The given apparatus (e.g. HPLC system) might serve as example among other possibilities. For your convenience, an example HPLC method for the analysis of T2- toxin is given below.
Sample Preparation (Literature Method Given):
Samples which content of T2/HT2 are to be analyzed, e.g. cereals, are extracted by the method of Visconti et al.1 using methanol-water (90/10 v/v) as extraction solvent. E.g. to 50g of sample are added a volume of 100ml of the extraction solvent and processed as cited.