SeroELISA Chlamydia IgG Assay Kit

$395.00

This Eagle Biosciences SeroELISA Chlamydia IgG Assay Kit is intended for the determination of specific IgG antibody to Chlamydia in a single human serum sample or to evaluate paired sera, by an Enzyme-Linked Immunosorbent Assay (ELISA). This SeroELISA Chlamydia IgG Assay is for research use only and not for use in diagnostic procedures.

SKU: 111-01 Categories: , ,

SeroELISA Chlamydia IgG Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: Cut-Off Control
Incubation Time: 2 hours
Sample Type: Serum
Sample Size: 10 µL

Additional Information

Assay Background


Chlamydia is a gram-negative obligate intracellular bacteria that causes acute and chronic diseases in mammalian and avian species. The genus Chlamydia is comprised of four species: C.trachomatis, C.pneumoniae, C.psittaci and C. pecorum. C.trachomatis is divided into 15 serovars. Serovars A, B, Ba and C are agents of trachoma, the leading cause of preventable blindness endemic in third world countries. Serovars L1-L3 are the agents of lymphogranuloma venereum. Serovars D-K are the common cause of sexually transmitted genital infection worldwide: cervicitis, endometritis/ salpingitis in females and urethritis (4) in both males and females. Endometritis/salpingitis can lead to tubal occlusion with a higher risk of extra uterine pregnancy and infertility. Genital infection may cause an acute and persistent infection occasionally without any clinical symptoms. Generally, these infections are treatable once they are diagnosed. However, without any treatment the infection may progress to a severe chronic inflammation leading to infertility, ectopic pregnancy, induced abortion or premature delivery. Moreover, infants to infected mothers may be infected during birth, leading to conjunctivitis or pneumonia. C.pneumoniae is an important respiratory pathogen in humans and causes up to 10% of community-acquired pneumonia cases. It has been associated with acute respiratory diseases, pneumonia, asthma, bronchitis, pharyngitis, acute chest syndrome of sickle cell disease, coronary heart disease, and Guillain-Barre syndrome. C.psittaci infects a diverse range of host species from mollusks to birds to mammals and also causes severe pneumonia.

Serodiagnostic tests, which rely on specific immunologic markers, serve as a non-invasive diagnostic tool in identification of both distal and deep infections. It has been found that elevated titers of anti-Chlamydia IgG antibody are indicative of active chlamydial infection. High levels of anti-Chlamydia IgG antibody are of diagnostic value in chronic or systemic infections such as salpingitis, mechanical infertility, perihepatitis, epididymitis, Reiter’s syndrome and pneumonitis (3, 9, 10). The SeroELISA Chlamydia test employs the L2 serovar broadly reacting antigen of C. trachomatis. It will detect C. trachomatis, C. psittaci and C. pneumoniae (TWAR) antibodies

Assay Principle


Human serum to be tested is brought into contact with the antigenic material coating the microtiter wells. Specific antibody, if present in the patient serum will bind to the attached antigen, a complex is formed and all the serum components are washed away in the wash phase. Horseradish peroxidase (HRP) conjugated anti-human IgG ( chain specific) is added to the wells. If an antigen-antibody complex was formed in the previous step, the peroxidase-conjugated antibody will bind to the antibody moiety of the complex. If no antigen-antibody complex was formed in the previous step, the conjugate is washed away in the wash phase. TMB-Substrate is added. A positive reaction is indicated by a blue to deep blue color which develops in the test wells following enzymatic reaction of the peroxidase moiety with peroxide and the chromogen reactant. After the enzymatic reaction is stopped by an acidic solution. The absorbance of the test wells is determined at 450nm by a spectrophotometer. The absorbance at 450nm is indicative of IgG anti-Chlamydia titer in patient serum specimens.

Cross Reaction


Hospitalized patients, infected with Neisseria gonorrhea, Staphylococcus aureus and Peptostreptococcus anaerobius, who were diagnosed by commercial serology kits, were also tested with the SeroELISA Chlamydia kit. There was no significant cross-reaction detected.

Manual

Product Manual


References

References


  1. Yuan, Y., Zhang, Y. X., Watkins, N. G. and Caldwell, H.D. (1989). Nucleotide and Deduced Amino Acid Sequences for the Four Variable Domains of the Major Outer Membrane Proteins of the 15 Chlamydia trachomatis Serovars. Infection and Immunity. 57:1040-1049. Copyright 1989, American Society for Microbiology.
  2. Treharne J. D. (1985). The community epidemiology of trachoma. Rev Infect Dis. 7:760-763.
  3. Piura, B., Sarov, I., Sarov, B., Kleinman, D., Chaim, W. and Insler, V. (1985). Serum IgG and IgA antibodies specific for    Chlamydia trachomatis in salpingitis patients as determined by the immunoperoxidase assay. Eur. J. Epidemiol 1: 110-116.
  4. Wang, S.P., Grayston, J.T., Kuo, C.C., Alexander, E.R., and Holmes, K.K. (1977). SeroDiagnosis of Chlamydia trachomatis infection with the microimmunofluorescence test. In: Nongonoccolcal urethritis and related infection, D. Hobson and K.K. Holmes (Eds), P. American Society for Microbiology, Washington DC. p. 237-248.
  5. Thompson III S. E., and Dretler R. H. (1982). Epidemiology and Treatment of Chlamydial Infections in Pregnant Women and Infants. Review of Infectious Diseases 4:S747
  6. Saikku P., Mattila, K., Nieminen, M.S., Huttunen, J.K., Leinonen, M., Ekman, M.R., Makela, P.H., and Valtonen, V. (1988). Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infection. Lancet II: 983-986.
  7. Sarov, I., Kleinman, D., Cevenini R., Holcber, G., Potashnik, G. Sarov, B . and Insler, V. (1986). Specific IgG and IgA Antibodies to Chlamydia trachomatis in Infertile Women. Int. J. Fertil 31:193-197.
  8. Csango, P.A., Sarov, B., Schiotz, H., Sarov, I. (1988) Comparison Between Cell Culture and Serology for Detecting Chlamydia trachomatis in women seeking abortion. J. Clin. Pathol. 41:89-92.
  9. Tsunekawa, t. and Kumamoto, Y. (1989). A Study of IgA and IgG Titers of C.trachomatis in Serum and Prostatic Secretion in Chronic Prostatitis. J. J. A. Inf. Dis. 63:130-137.
  10. Kletter, Y., Caspi. D., Yarom, M., Sarov, B., Sarov, I. And Tanay, A. (1988) Serum IgA and IgG Antibodies Specific to Chlamydia in Patient’s with Reiter’s Syndrome (RS). In: Proceedings of the European Society for Chlamydia Research, Societa Editrice Esculapio, Bologna. p. 170.