S-Adenosyl-L-Methionine (SAM) ELISA Assay Kit

$1,460.00

The Eagle Biosciences S-Adenosyl-L-Methionine (SAM) ELISA Assay Kit is intended for the quantitative determination of S-Adenosyl-L-Methionine (SAM) in serum or plasma. The Eagle Biosciences S-Adenosyl-L-Methionine (SAM) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

SKU: SAM31-K01 Categories: , ,

S-Adenosyl-L-Methionine (SAM) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: 30 nM
Dynamic Range: 30 – 960 nM
Incubation Time: 1.5 hours
Sample Type: Serum, Plasma
Sample Size: 30 µL

Control Included

Additional Information

Assay Background

Methionine is an amino acid with sulfur methyl group, which is converted into S-adenosylmethionine (SAM) with ATP by methionine adenosyltransferase (EC 2.5.1.6). SAM is the sole methyl group donor for over 50 kinds of biologically active substances such as DNA, RNA, protein, phospholipids, hormones and neuro-transmitters. After methyl group is transferred away from SAM under a mehtyltransferase, S-adenosylhomocysteine is formed, which is further metabolized into homocysteine (Hcy) after adenosine is removed. The homocysteine is metabolized to methionine through N5-methyl tetrahydrofolate methyltransferase (EC1.1.1.68) and coenzyme vitamin B12 catalytic pathway accepting methyl from N5-methyltetrahydrofolate. This is methionine cycle.

Methylation index is defined as the ratio of S-Adenosyl-L-Methionine (SAM) and S-Adenosyl-L-homocysteine (SAH).  Methylation index is a better marker for methylation status and methylation capability.  SAM is used as medicines or nutritional supplements for treating depression, liver diseases, arthritis and joint pain, etc.  This S-Adenosyl-L-Methionine (SAM) ELISA Immunoassay Kit is used to measure the level of SAM in serum or plasma samples of soluble form.

Assay Principle

This direct competitive S-Adenosyl-L-Methionine (SAM) ELISA Assay Kit (Enzyme Linked Immunosorbent assay) is designed to measure the level of S-adenosyl-L-methionine (SAM) in serum or plasma. SAM conjugated with macromolecule is immobilized on the micro-titer plate. Standards and samples are pipetted into the wells, and then the HRP-conjugated antibody against SAM is added. The free SAM molecule in samples or standards competes with the immobilized SAM on the micro-titer plate surface for binding sites of the antibody. After discarding the mixed solution and washing each well, TMB substrate solution is added. The substrate solution turns blue under the effect of HRP (horseradish peroxidase), and changes into yellow once stop solution (acid) is added. The color develops in inverse proportion to the amount of SAM in the sample (or standards). The optical density of the remaining solution (OD450) is measured at 450nm using micro-plate spectrophotometer. The level of SAM in samples can be calculated through standard curve generated with standards.

Manual

Product Manual


Publications

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