RNase 7 ELISA Assay
The RNase 7 ELISA Assay is For Research Use Only
Size: 1×96 wells
Sensitivity: 1 pg/ml
Dynamic Range: 10 – 640 pg/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatant
Sample Size: 100 µL
Alternative Names: hRNase 7, Ribonuclease A Family Member 7
Assay Principle
This human RNase 7 ELISA test kit is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with human RNase 7-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human RNase 7 molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human RNase 7-specific monoclonal detection antibodies bind to a different epitope of human RNase 7 molecules. After washing, the ready–to-use HRP substrate (TMB) is added to the wells. The intensity of the colour produced is directly proportional to the amount of human RNase 7 in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.
Related Products
Human Recombinant RNase 7 Protein
MANF ELISA Assay Kit
CDNF ELISA Assay Kit
Product Developed and Manufactured by Icosagen