Immunology ELISA Assay Kit

RNase 7 ELISA Assay

$980.00

Th RNase 7 ELISA Assay Kit is intended for the quantitative determination of human RNase 7 (ribonuclease 7) (all three natural variants) in biological fluids (urine, saliva, skin lavage etc.) and cell culture supernatant. The RNase 7 ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures. The kit has been developed based on RNase 7 antibodies, which are raised against full-length recombinant canonical form of hRNase 7.

SKU: RNA31-K01 Categories: , ,

RNase 7 ELISA Assay

The RNase 7 ELISA Assay is For Research Use Only

Size: 1×96 wells
Sensitivity: 1 pg/ml
Dynamic Range: 10 – 640 pg/mL
Incubation Time: 2.5 hours
Sample Type: Serum, Plasma, Cell Culture Supernatant
Sample Size: 100 µL
Alternative Names: hRNase 7, Ribonuclease A Family Member 7

Assay Principle

This human RNase 7 ELISA test kit is based on the quantitative sandwich enzyme immunoassay technique. Microtiter wells are pre-coated with human RNase 7-specific monoclonal capture antibodies. Samples and standards are pipetted into microwells and human RNase 7 molecules present in the sample are bound by the capture antibodies. After incubation, unbound material is removed by washing the wells. Then, horseradish peroxidase (HRP) conjugated human RNase 7-specific monoclonal detection antibodies bind to a different epitope of human RNase 7 molecules. After washing, the ready–to-use HRP substrate (TMB) is added to the wells. The intensity of the colour produced is directly proportional to the amount of human RNase 7 in the sample. Colour development is then stopped by the addition of stop solution. Absorbance is measured at 450 nm.

Related Products

Human Recombinant RNase 7 Protein
MANF ELISA Assay Kit
CDNF ELISA Assay Kit
Product Developed and Manufactured by Icosagen

Additional Information

Assay Procedure

  1. Dilute 50 mL wash concentrate with 450 mL of distilled water.
  2. Prepare the standards by serial dilutions using the stock standard and the sample diluent (pink).
  3. Perform the dilution of each sample in the sample diluent.
  4. Add 100 μL samples and standards into appropriate wells in duplicate.
  5. Incubate the covered microplate for 60 min at RT on a microwell plate shaker (300 rpm).
  6. Remove the liquid and wash the wells 4 times with 300 μL of washing solution.
  7. Add 100 μL of enzyme conjugate (blue) into each well. STEP 8
  8. >Incubate the covered microplate for 60 min at RT on a microwell plate shaker (300 rpm).
  9. Remove the liquid and wash the wells 4 times with 300 μL of washing solution.
  10. Add 100 μL of substrate solution into each well.
  11. Incubate the covered microplate for 20 minutes at RT on a microwell plate shaker (300 rpm).
  12. Stop the reaction by adding 50 μl of Stop solution into each well in the same order and time as for TMB distribution.
  13. Read the absorbance at 450 nm immediately.

Typical Standard Curve

RNase 7 ELISA Assay

Manual

Product Manual