RBM3 ELISA Assay Kit

$820.00

The RBM3 ELISA Assay Kit provides a fast, highly sensitive and specific quantitative of RNA-binding protein 3 (RBM3) in human serum or plasma samples. Do not use for any other biological sample. One kit contains reagents for 96 determinations, thus allowing the measurement of one standard curve and 40 samples in duplicate. The Human RBM3 ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

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RBM3 ELISA Assay Kit

The RBM3 ELISA Assay Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 10 pg/mL
Dynamic Range: 31 to 2000 pg/ml
Incubation Time: 2.5 hours
Sample Type: Serum and Plasma
Alternative Name: RNA-Binding Protein 3 ELISA, Human RBM3 ELISA


Assay Background

The RNA-binding protein 3 (RBM3) is a member of the glycine-rich RNA-binding protein family. The exact role of the cold shock protein RBM3 is not yet clear. The role of mRNA-binding proteins can be attributed to a regulation of mRNA splicing, stability, transport and ultimately the translation of mRNA into proteins. RBM3 consists of 157 amino acids with a predicted mass of 17 kD and is one of the few proteins that are upregulated at low temperature. It is known that RBM3 acts especially in cellular stress (eg. hypothermia) as mRNA chaperones and protein translation stabilizer. Chip et al. were able to show that RBM3 has anti-apoptotic properties in neurons and recent studies show that RBM3 mediates structural plasticity and protective effects of neurodegeneration. For these reasons, RBM3 may play an important role as biomarker for many neurodegenerative diseases and could be used to monitor the effectiveness of therapeutic hypothermia. Several studies report RBM3 as prognostic marker in cancer or in linking stress-regulated RNA splicing to tumorgenesis. Low nuclear expression of RBM3 has been found to be associated with poor prognosis in several cancer forms e.g. breast, ovarian, colorectal, prostate cancer and malignant melanoma.


Sample Preparation and Storage:
Serum or plasma samples (EDTA or Heparin as anticoagulant) may be stored at -20°C or -80°C. When stored at -20°C or -80°C, it is absolutely necessary to mix the samples thoroughly prior to measuring. Avoid freeze-thaw cycles. Dilute the serum or plasma samples minimum 1:10 with RBM3-SAMPLE-BUF, depending on the possible concentration of the analyte.


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Additional Information

Assay Principle


The Eagle Biosciences Human RBM3 ELISA (Enzyme-Linked Immunosorbent Assay) Assay Kit is an immunological quantitative detection method based on a sandwich ELISA.

The Eagle Biosciences Human RBM3 ELISA Assay Kit is performed in a 96-well microplate. The wells of the microplate are pre-coated with a specific capture antibody against human RBM3 that binds free RBM3 from the patient sample. In one step simultaneous incubation of the RBM3 standards or samples and of the detection conjugate (peroxidase conjugate of an anti-RBM3 antibody) is carried out. Incubation takes place 2 hours at 20-25°C. After a washing step, the colour substrate TMB (tetramethylbenzidine) is added to the wells. The reaction is stopped by adding the Stop Solution and the yellow colour is read in a microtiter plate reader at 450 nm. The concentration of RBM3 in a sample is determined by interpolation from the standard curve.

Manual

Product Manual


Publications

References


  1. Danno, S., et al., Increased transcript level of RBM3, a member of the glycine-rich RNA-binding protein family, in human cells in response to cold stress. Biochemical and Biophysical Research Communications, 1997. 236(3): p. 804-807<
  2. Derry, J.M., J.A. Kerns, and U. Francke, RBM3, a novel human gene in Xp11. 23 with a putative RNA- binding domain. Human molecular genetics, 1995. 4(12): p. 2307-2311.
  3. Dresios, J., et al., Cold stress-induced protein Rbm3 binds 60S ribosomal subunits, alters microRNA levels, and enhances global protein synthesis. Proceedings of the National Academy of Sciences of the United States of America, 2005. 102(6): p. 1865-1870.
  4. Nishiyama, H., et al., Cloning and characterization of human CIRP (cold-inducible RNA-binding protein) cDNA and chromosomal assignment of the gene. Gene, 1997. 204(1-2): p. 115-120<
  5. Smart, F., et al., Two isoforms of the cold‐inducible mRNA‐binding protein RBM3 localize to dendrites and promote translation. Journal of Neurochemistry, 2007. 101(5): p. 1367-1379.
  6. Danno, S., et al., Increased transcript level of RBM3, a member of the glycine-rich RNA-binding protein family, in human cells in response to cold stress. Biochemical and Biophysical Research Communications, 1997. 236(3): p. 804-807.
  7. Chip, S., et al., The RNA-binding protein RBM3 is involved in hypothermia induced neuroprotection. Neurobiology of Disease, 2011. 43(2): p. 388-396.
  8. Peretti, D., et al., RBM3 mediates structural plasticity and protective effects of cooling in neurode- generation. Nature, 2015. 518: p.236-239
  9. Zeng, Y., Stress-response protein RBM3 attenuates the stem-like properties of prostate cancer cells by interfering with CD44 variant splicing. 2013.73 (13): p.4123-33
  10. Olofsson, S., et al., Low RBM3 protein expression correlates with clinical stage, prognostic classification and increased risk of treatment failure in testicular non-seminomatous germ cell cancer. PLoSOne. 2015. 10 (3): e0121300
  11. Ehlen, A., et al., Expression of the RNA-binding protein RBM3 is associated with a favourable prognosis and cisplatin sensitivity in epithelial ovarian cancer. J. Trans. Med., 2010, 8:78
  12. Boman, K., Decreased expression of RNA-binding motif protein 3 correlates with tumour progression and poor prognosis in urothelial bladder cancer. BMC Urol. 2013. 8: 13-17
  13. Nodin, B., et al., High MCM3 expression is an independent biomarker of poor prognosis and correlates with reduced RBM3 expression in a prospective cohort of malignant melanoma. Diagn. Pathol. 2012 (7): 82