RBM3 ELISA Assay Kit
The RBM3 ELISA Assay Kit is For Research Use Only
Size: 1×96 wells
Sensitivity: 10 pg/mL
Dynamic Range: 31 to 2000 pg/ml
Incubation Time: 2.5 hours
Sample Type: Serum and Plasma
Alternative Name: RNA-Binding Protein 3 ELISA, Human RBM3 ELISA
The RNA-binding protein 3 (RBM3) is a member of the glycine-rich RNA-binding protein family. The exact role of the cold shock protein RBM3 is not yet clear. The role of mRNA-binding proteins can be attributed to a regulation of mRNA splicing, stability, transport and ultimately the translation of mRNA into proteins. RBM3 consists of 157 amino acids with a predicted mass of 17 kD and is one of the few proteins that are upregulated at low temperature. It is known that RBM3 acts especially in cellular stress (eg. hypothermia) as mRNA chaperones and protein translation stabilizer. Chip et al. were able to show that RBM3 has anti-apoptotic properties in neurons and recent studies show that RBM3 mediates structural plasticity and protective effects of neurodegeneration. For these reasons, RBM3 may play an important role as biomarker for many neurodegenerative diseases and could be used to monitor the effectiveness of therapeutic hypothermia. Several studies report RBM3 as prognostic marker in cancer or in linking stress-regulated RNA splicing to tumorgenesis. Low nuclear expression of RBM3 has been found to be associated with poor prognosis in several cancer forms e.g. breast, ovarian, colorectal, prostate cancer and malignant melanoma.
Sample Preparation and Storage:
Serum or plasma samples (EDTA or Heparin as anticoagulant) may be stored at -20°C or -80°C. When stored at -20°C or -80°C, it is absolutely necessary to mix the samples thoroughly prior to measuring. Avoid freeze-thaw cycles. Dilute the serum or plasma samples minimum 1:10 with RBM3-SAMPLE-BUF, depending on the possible concentration of the analyte.