Rat GRP78 ELISA Assay Kit

$650.00$2,590.00

The Rat GRP78 ELISA Assay Kit is a sandwich ELISA that can be used for the quantification of Rat GRP78 in Tissue Lysates. The ELISA utilizes an Anti-Rat GRP78 monoclonal antibody-coated plate and a Biotinylated antibody for detection which allows for an assay range of 0 to 50 ng/mL, with a sensitivity of 0.15 ng/mL. The other highlights of this kit are a quick incubation time, stable reagents, and an easy to use protocol.

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SKU: SKT-137 Categories: , ,

Rat GRP78 ELISA Assay Kit

For Research Use Only

Sensitivity: 0.15 ng/ml
Dynamic Range: 0 – 50 ng/ml
Incubation Time: 30 minutes
Sample Type: Tissue
Sample Size: 100 μL

Product manufactured in Canada by StressMarq.

Additional Information

Assay Background


GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5’ nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti­tumor drugs (6, 7).

Assay Procedure


  1. Prepare Standard and samples in Standard and Sample Diluent.
  2. Add 100 µL of Standard or sample to appropriate wells.
  3. Cover plate with Plate Sealer and incubate at 37°C for 2 hours.
  4. Wash plate four times with 1X Wash Buffer.
  5. Add 100 µL of Biotinylated Antibody Working Solution to each well.
  6. Cover plate with Plate Sealer and incubate at at 37°C for 2 hours.
  7. Wash plate four times with 1X Wash Buffer.
  8. Add 100 µL of Streptavidin Poly HRP Working Solution to each well.
  9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes.
  10. Wash plate four times with 1X Wash Buffer.
  11. Add 100 µL of TMB Substrate to each well.
  12. Develop the plate in the dark at room temperature for 30 minutes.
  13. Stop reaction by adding 100 µL of Stop Solution to each well.
  14. Measure absorbance on a plate reader at 450 nm.

Typical Standard Curve


Manual

Product Manual