Prostaglandin E2 EIA Kit


The Prostaglandin E2 EIA Kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples.

SKU: SKT-207-96 Categories: , ,

Prostaglandin E2 EIA Kit

The Prostaglandin E2 EIA Kit is For Research Use Only

Size: 1×96 wells
Sensitivity: 3.07 pg/ml
Dynamic Range: HS: 1.953 – 500 pg/ml. LV: 15.625 – 1000 pg/ml. RG: 3.906 – 500 pg/ml. ON: 3.906 – 500 pg/ml.
Incubation Time: 30 minutes
Sample Type: EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Culture Media, Urine
Sample Size: 50 μL

Product manufactured in Canada by StressMarq.

Assay Background

Eicosanoid signal transduction pathways are highly conserved and are involved in a number of physiological processes. Prostaglandins are synthesized from arachidonic acid by cyclooxygenase (COX)-1 or -2, which convert the acid into PGH2. This is further processed by cytosolic or microsomal prostaglandin synthases to become PGE2 or one of several other prostanoids. Prostacyclin is the major cyclooxygenase product in blood vessel walls and it is present in inflammatory fluids in similar concentrations to PGE2. Prostacyclin is a potent vasodilator and is more potent than PGE2 in producing hyperalgesia. PGE2 is produced by a wide variety of tissues and in several pathological conditions, including inflammation, arthritis, fever, tissue injury, endometriosis, and a variety of cancers. Other biological actions of PGE2 include vasodilation, modulation of sleep/wake cycles, and facilitation of human immunodeficiency virus replication. It elevates cAMP levels, stimulates bone resorption, and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics.

The unopened kit should be stored at -20°C. Once opened the kit can be stored at 4°C up to the expiration date on the kit label, except for the PGE2 Standard and PGE2 Conjugate. These must be stored at -20°C. The frozen PGE2 Conjugate can be freeze-thawed multiple times.

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Additional Information

Typical Standard Curve

Prostaglandin E2 EIA Kit