PCSK9 ELISA Assay Kit

$820.00

The Human PCSK9 ELISA Assay Kit is intended for the sensitive and specific quantitative determination of PCSK9 or Proprotein Convertase Subtilisin / Kexin Type 9 in human serum samples. The Human PCSK9 ELISA Assay Kit is for research use only and not to be used for diagnostics procedures.

SKU: BTPCSK9-001 Categories: , , , Tag:

PCSK9 ELISA Assay Kit

The PCSK9 ELISA Assay Kit is For Research Use Only

Size: 1x96wells
Sensitivity: 1 ng/ml
Dynamic Range: 5.27 ng/ml to 60 ng/ml
Incubation Time: 2 hours 20 minutes
Sample Type: Serum
Sample Size: 100 µL
Alternative Names: Proprotein Convertase Subtilisin / Kexin Type 9, PCSK9

Controls Included


Assay Background

Proprotein Convertase Subtilisin / Kexin Type 9 (PCSK9) has importance because it interferes with the lipoprotein in particular the lipoprotein particles (LDL) homeostasis. PCSK9 binds to the lipoprotein particles receptor (LDLR) which transports fat molecules within the extracellular fluid. After LDL binding to the LDL receptor the complex of LDLR/PCSK9/LDL gets degraded in the hepatocytes upon internalization. Importantly if PCSK9 does not bind to the complex, the LDLR can return to the surface of the cell and can thereby continue to remove LDL particles from the bloodstream.

Agents which block PCSK9 can lower LDL particle concentrations in the blood. The therapeutical antibody against PCSK9 Evolocumab, was approved by the U.S. Food and Drug Administration in 2015 for lowering LDL particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.


Storage and Sample Preparation

Storage

  • All components of the PCSK9 ELISA Assay kit should be stored in the refrigerator (2-8 °C).
  • Once reconstituted, the PCSK9-Standard-CONC should be used immediately or stored at -80 °C.
  • The dilutions of BIOT-ANTIBODY-CONC and STAV-CONJ-CONC should be prepared freshly directly before use.
  • When running a partial plate, only suitable aliquots of these solutions should be made.
  • After dilution WASHBUF is stable for 4 weeks at storage temperature 2-8°C.

Serum Preparation and Storage

  • Serum may be stored at -80°C. When stored at -80°C, it is absolutely necessary to mix the samples thoroughly prior to measuring with the ELISA. Avoid freeze-thaw cycles.
  • Dilute the serum samples minimum 1:20 with PCSK9-SAMPLE-BUF, depending on the possible concentration of the analyte.

 


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Additional Information

Assay Background


The Human PCSK9 ELISA (Enzyme-Linked Immunosorbent Assay) is an immunological quantitative detection method based on a sandwich ELISA.

The ELISA is performed in a 96-well microplate. The wells of the microplate are pre-coated with a specific capture antibody against human PCSK9 that binds free PCSK9 from the patient sample. The analyte is detected in two steps using a biotin-labelled monoclonal antibody and a highly polymerised streptavidin-peroxidase conjugate. The incubation steps take place for 45 min/20 min at 20-25°C. After a washing step, the colour substrate TMB (Tetramethylbenzidine) is added to the wells. The reaction is stopped by adding the stop solution and the yellow colour is read in a microtiter plate reader at 450 nm. The concentration of PCSK9 in a sample is determined by interpolation from the standard curve.


Standard Curve

Documents

Product Manual


Please note: All documents above are for reference use only and should not be used in place of the documents included with this physical product. If digital copies are needed, please contact us.

Product Citations