Osteoprotegerin (OPG) ELISA Assay Kit


The Eagle Biosciences Human Osteoprotegerin (OPG) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human osteoprotegerin levels in serum or plasma samples. The Eagle Biosciences Human Osteoprotegerin (OPG) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

SKU: BI-20403 Categories: , ,

Osteoprotegerin (OPG) ELISA Assay Kit

For Research Use Only

Size: 1×96 wells
Sensitivity: LOD: (0 pmol/l + 3 SD): 0.07 pmol/l; LLOQ: 0.08 pmol/l
Dynamic Range: 0 to 20 pmol/l
Incubation Time: 5.5 hours
Sample Type: Serum, plasma (EDTA, heparin, citrate)
Sample Size: 20 µL
Controls Included

Product Developed and Manufactured in Austria by Biomedica

Unit conversion: 1 pg/ml = 0.05 pmol/l (MW: 19.9 kD)

Additional Information

Assay Background

Osteoprotegerin (OPG) or Osteoclast inhibitory factor (OCIF) is a glycoprotein of the TNF receptor superfamily 11b (gene name TNFRSF11B) https://www.uniprot.org/uniprot/O00300. OPG is synthesized as a monomer of 380 amino acids and is assembled as a homodimer within the cell and then secreted mainly as a disulfide-linked homodimer into the extracellular compartment. OPG is produced by many different tissues and cell types including osteoblasts. OPG is a negative regulator of bone resorption by acting as decoy receptor for RANKL, thus neutralizing its function in osteoclastogenesis. This glycoprotein is also involved in the regulation of vascular calcification.

Assay Procedure

  1. Pipette 150 µl ASYBUF (Assay Buffer, red cap) into each well.
  2. Add 20 µl STD/SAMPLE/CTRL (Standard/Sample/Control) in duplicate into respective wells, swirl gently.
  3. Add 50 µl AB (biotinylated anti OPG antibody, green cap) into each well, swirl gently.
  4. Cover tightly and incubate for 4 hours at room temperature (18-24°C).
  5. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer). After final wash, remove remaining WASHBUF by strongly tapping plate against paper towel.
  6. Add 200 µl CONJ (Conjugate, amber cap) into each well.
  7. Cover tightly and incubate for 1 hour at room temperature (18-24°C).
  8. Aspirate and wash wells 5x with 300 µl diluted WASHBUF (Wash buffer). After final wash, remove remaining WASHBUF by strongly tapping plate against paper towel.
  9. Add 200 µl SUB (Substrate, blue cap) into each well.
  10. Incubate for 30 min at room temperature (18-24°C) in the dark.
  11. Add 50 µl STOP (Stop solution, white cap) into each well, swirl gently.
  12. Measure absorbance immediately at 450 nm with reference 630 nm, if available.


Typical Standard Curve


Expected Values

Values from apparently healthy individuals:

  • Median serum (n=60): 2.7 pmol/l
  • Median EDTA plasma (n=6): 2.2 pmol/l
  • Median heparin plasma (n=7): 2.3 pmol/l
  • Median citrate plasma (n=5): 2.3 pmol/l

It is recommended to establish the normal range for each laboratory.


Product Manual



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