Normetanephrine & Metanephrine (Urine) ELISA Assay Kit

$530.00

The Eagle Biosciences Normetanephrine and Metanephrine (Urine) ELISA Assay Kit is intended for the quantitative determination of normetanephrine and metanephrine in urine by a microplate enzyme immunoassay. The Normetanephrine and Metanephrine (Urine) ELISA Assay Kit is for research use only and not to be used in clinical, therapeutic or diagnostic procedures.

Normetanephrine & Metanephrine (Urine) ELISA Assay Kit

For Research Use Only

Size: 2 x 96 wells
Sensitivity:  Metanephrine: 4 ng/ml, Normetanephrine: 10 ng/ml
Dynamic Range:  Metanephrine: 20 – 2400 ng/ml, Normetanephrine: 30 -4000 ng/ml
Incubation Time: 3 hours
Sample Type: Urine (Also available for Plasma)
Sample Size: 10 µL

Controls Included

Product Developed and Manufactured in Germany with SKU EA614/92 by DLD Diagnostika


Additional Information

Assay Background

Normetanephrine and metanephrine are physiologically formed from the catecholamines noradrenaline and adrenaline by the enzyme catechol-O-methyltransferase (COMT). Increased levels of normetanephrine and metanephrine can be found in pheochromocytoma, ganglioneuroma and other neurogenic tumors. The Normetanephrine and Metanephrine (Urine) ELISA assay kit provides materials for the quantitative measurement of normetanephrine and metanephrine in urine. Normetanephrine and metanephrine are quantitatively acylated to their N-acyl-derivates.

Assay Principle

The competitive Normetanephrine and Metanephrine (Urine) ELISA Assay kit uses the microtiter plate format. Metanephrine and normetanephrine, respectively, are bound to the solid phase of the microtiter plate. Acylated nor-/metanephrines from the sample and solid phase bound nor-/metanephrines compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase nor-/metanephrine is detected by anti-rabbit IgG/peroxidase. The substrate TMB/peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase nor-/metanephrines is inversely proportional to the nor-/metanephrines concentration of the sample.

Manual

Product Manual


Publications

Citations

Nizar, JM;Dong, W;McClellan, RB;Labarca, M;Zhou, Y;Wong, J;Goens, DG;Zhao, M;Velarde, N;Bernstein, D;Pellizzon, M;Satlin, LM;Bhalla, V; Sodium-Sensitive Elevation in Blood Pressure is ENaC Independent in Diet-Induced Obesity and Insulin Resistance, Am. J. Physiol. Renal Physiol., ajprenal.00265.2015, DOI:10.1152/ajprenal.00265.2015