MutaGEL r-Vitamin D3 PCR Assay


The MutaGEL® r-Vitamin D3 test kit allows the detection of the codon changing DNA variability (start codon polymorphism) in the gene of vitamin D3 receptor (VD3R) which encodes for the corresponding protein involved in calcium metabolism. The MutaGEL® r-Vitamin D3 is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09004 Categories: ,

MutaGEL r-Vitamin D3 PCR Assay

MutaGEL r-Vitamin D3 PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only

Controls Included

Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the “VD3R positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).

Assay Principle

The MutaGEL® r-vitamin D3 PCR Assay contains a set of primers for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of varying genotypes (start codon polymorphism) is characterized by subsequent specific restriction enzyme digestion. The rare variant (f, pathogenic) possesses a restriction site for the specific endonuclease, whereas the major variant (F, protective) does not. The amplified products obtained from the pathogenic DNA variant will, therefore, be cut into two fragments, whereas the protective DNA variant will not be cut. The identification of the existing genotype from the patient is done by subsequent analysis of the DNA fragments by gel-electrophoretic methods (Dr. Essrich, Biologisches Labor, Denzlingen).

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Learn more about Vitamin D at Eagle Biosciences Biomarker Spotlight page dedicated to Vitamin D here:

Vitamin D Biomarker Spotlight

Additional Information

Assay Background

Vitamin D3 (cholecalciferol) receptor is a protein involved in the regulation of blood calcium levels and also in bone turnover processes. Sequence analysis detected a frequently amino acid changing variability in the vitamin D3 receptor gene (due to a second ATG triplet three codons before). This start codon polymorphism alters vertebral bone fracture frequency of elderly persons, although in different populations to a different extent.


Product Manual


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