MutaGEL r-Vitamin D3 PCR Assay
MutaGEL r-Vitamin D3 PCR Assay manufactured in Germany by Immundiagnostik
Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the “VD3R positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).
The Eagle Biosciences kit MutaGEL® r-vitamin D3 contains a set of primers for amplification of the specific DNA sequence within the human vitamin D3 receptor gene VD3R. Amplificates of varying genotypes (start codon polymorphism) is characterized by subsequent specific restriction enzyme digestion. The rare variant (f, pathogenic) possesses a restriction site for the specific endonuclease, whereas the major variant (F, protective) does not. The amplified products obtained from the pathogenic DNA variant will, therefore, be cut into two fragments, whereas the protective DNA variant will not be cut. The identification of the existing genotype from the patient is done by subsequent analysis of the DNA fragments by gel-electrophoretic methods (Dr. Essrich, Biologisches Labor, Denzlingen).