MutaGEL r-Calcitonin PCR Assay

$750.00

The Eagle Biosciences MutaGEL® r-Calcitonin test kit allows the detection of the codon changing DNA variability (C→T mutation, Pro463Leu polymorphism) in the gene of calcitonin receptor (CTR) which encodes for the corresponding protein involved in bone metabolism. The MutaGEL® r-Calcitonin is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09003 Categories: ,

MutaGEL r-Calcitonin PCR Assay Kit

MutaGEL r-Calcitonin PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only

Controls Included


Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the “CTR positive control DNA” for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).


Assay Principle

The Eagle Biosciences MutaGEL® r-Calcitonin kit contains a set of primer for amplification of the specific sequence within the human calcitonin receptor gene CTR. Amplificates of varying genotypes (C→T mutation, Pro463Leu polymorphism) is characterized by subsequent specific restriction enzyme digestion.  The variant (C, protective) possesses a restriction site for the endonuclease whereas the other variant (T, pathogen) does not. The amplified products obtained from the protective DNA-variant will, therefore, be cut into two fragments, whereas the pathogen DNA-variant will not be cut. The identification of the present genotype is done by analysis of present DNA-fragments by gel electrophoretic methods (Dr. Essrich, Biologisches Labor, Denzlingen).


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Additional Information

Assay Background


Calcitonin receptor is a membrane protein produced by osteoclasts and it functions as a transmitter of the peptide hormone calcitonin leading to decreased bone resorption processes. Sequence analysis detected a frequently amino acid changing variability in the receptor gene. This polymorphism alters the bone density and fracture frequency if healthy persons are compared with osteoporotic patients or even between populations with different skeletal measurement. It could be shown that the polymorphism changes a phosphorylation region of the 4th intracellular domain. By this, the average more frequent allele “T” (Leucin) in Caucasians protects from the described pathogen effect (due to the rarer “C” allele (Prolin). Furthermore, it can be detected by specific restriction digest due to the creation of a new restriction site in the gene.

Manual

Product Manual