MutaGEL Prothrombin PCR Assay

$775.00

The MutaGEL Prothrombin Factor II PCR Assay allows the detection of the G20210A mutation within the 3’ UTR region of the prothrombin gene (human coagulation factor II). This mutation is related to a big amount of prothrombin in plasma. The MutaGEL® Prothrombin (Factor II) is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09022 Categories: ,

MutaGEL Prothrombin PCR Assay

MutaGEL Prothrombin PCR Assay manufactured in Germany by Immundiagnostik

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL
For Research Use Only
Controls Included


Storage and Stability
Storage at < -18°C. Reagents are stable in unopened micro tubes until the expiration date indicated. In general, avoid several thawing/freezing – cycles for the reagents. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment). Restrictions
The PCR results for positive control in DNA fragments of indicated length and for the samples at least one of both amplification products must be detectable. If this is not the case, the sample must be tested a second time or the complete analysis must be repeated with freshly isolated DNA. If there are no positive control DNA fragments present, the amplification was incorrect and the chosen PCR conditions have to been proven/ corrected.


Assay Principle

The MutaGEL® Prothrombin Factor II PCR Assay Kit contains a set of primers which amplify a specific sequence within the human factor II gene. The amplified product obtained from a wild-type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA homozygous for the factor II G20210A mutation will be cut. Amplification products and their cut fragments are analyzed through gel electrophoresis.


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Additional Information

Assay Background


Prothrombin (Factor II) is a protein in the blood required for the blood to clot. Blood clots are composed of a combination of blood platelets and a meshwork of the blood clotting protein fibrin. Prothrombin as blood clotting protein is needed to form fibrin. Persons with too little prothrombin have a bleeding tendency. If an individual has too much prothrombin, blood clots may form when they shouldn’t.

Manual

Product Manual