MutaGEL® Prothrombin (Factor II) PCR Assay


The Eagle Biosciences MutaGEL® Prothrombin PCR Assay allows the detection of the G20210A mutation within the 3’ UTR region of the prothrombin gene (human coagulation factor II). This mutation is related to a big amount of prothrombin in plasma. The MutaGEL® Prothrombin (Factor II) is for research use only and is not intended for diagnostic or therapeutic procedures.

SKU: KE09022 Categories: ,

MutaGEL® Prothrombin (Factor II) PCR Assay

For Research Use Only

Size: 24 Samples
Method: PCR (RFLP)
Sample Type: DNA (e.g.whole blood, cheek swab)
Sample Size: 200 µL

Product manufactured in Germany by Immundiagnostik

Additional Information

Assay Background

Prothrombin (Factor II) is a protein in the blood required for the blood to clot. Blood clots are composed of a combination of blood platelets and a meshwork of the blood clotting protein fibrin. Prothrombin as blood clotting protein is needed to form fibrin. Persons with too little prothrombin have a bleeding tendency. If an individual has too much prothrombin, blood clots may form when they shouldn’t.

Assay Principle

The Eagle Biosciences MutaGEL® Prothrombin PCR Assay Kit contains a set of primers which amplify a specific sequence within the human factor II gene. The amplified product obtained from a wild-type DNA will not be cut by the restriction enzyme included in this kit, whereas the fragment obtained from DNA homozygous for the factor II G20210A mutation will be cut. Amplification products and their cut fragments are analyzed through gel electrophoresis.


Product Manual