MutaGEL® Parodontitis PCR Assay
MutaGEL Parodontitis PCR Assay manufactured in Germany by Immundiagnostik
Size: 24 Samples
Method: PCR (allel specific)
Sample Type: DNA (sample from gingival pocket)
Sample Size: 200 µL
For Research Use Only
Assay Principle for MutaGEL Parodontitis PCR Assa
The MutaGEL Parodontitis PCR Assay contains different sets of primer which amplify each a specific sequence of the following pathogens: Porphyromonas gingivalis, Tannerella forsythensis, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans and Prevotella intermedia. Additionally, with primers for a highly conserved region of the bacterial 16S-RNA gene, an optical quantification of all contained bacteria can be done (analogous to the classical determination of germ numbers). In dependence of the diagnostic question the required primers for each individual case must be chosen before performance (provided Master Mix is enough to perform up to 96 PCRs and each pathogen specific primer mix allows 24 PCRs). Subsequently, the amplified pathogens are detected by classical gel electrophoresis (Pipetted in parallel. The generated amplicons have all different sizes). Development of this PCR method by Dr. Essrich, Biologisches Labor Freiburg/ Denzlingen (Germany).
Storage and Stability
Store at ≤ -18°C. The reagents are stable in the unopened micro tubes until the expiration date indicated (see print on the package). Do not thaw out the content of the positive control DNA for more than two times. If necessary, make suitable aliquots. Before use: Spin tubes briefly before opening (contents may become dispersed during shipment).